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Blood Hemoglobin Assay (Drabkins)

Protocols

BLOOD HEMOGLOBIN ASSAY (DRABKINS)   Drabkins Reagent Dissolve 1.0g NaHCO3, 0.05g KCN and 0.2...

Updated 2 years ago by Elliot Francis

BLOOD UROD

Protocols

BLOOD UROD   Sample preparation Draw two green-topped collection tubes of blood.  These tube...

Updated 2 years ago by Elliot Francis

Colorimetric PBG Assay (Ehrlich)

Protocols

COLORIMETRIC PORPHOBILINOGEN ASSAY (EHRLICH) Modified Ehrlich Reagent Dissolve 1.0 gm p-dimet...

Updated 2 years ago by Elliot Francis

COPOX Activity Assay

Protocols

COPROPORPHYRINOGEN III OXIDASE (COPOX) ASSAY Reaction A: Uroporphyrinogen III synthesis Add 8...

Updated 2 years ago by Elliot Francis

FECH Activity Assay

Protocols

FERROCHELATASE (FECH) Sample preparation Suspend ~50-µL mammalian cell pellet in 400µL TGD bu...

Updated 2 years ago by Elliot Francis

Hemin PPIX ZnPPIX Assay

Protocols

HEMIN, PROTOPORPHYRIN IX AND ZINC PROTOPORPHYRIN IX EHTYL ACETATE / ACETIC ACID EXTRACTION AND U...

Updated 2 years ago by Elliot Francis

PBGD Activity Assay

Protocols

PORPHOBILINOGEN DEAMINASE (PBGD)   PBGD Assay Add 10µL homogenized sample to 75µL in 0.1M Tr...

Updated 2 years ago by Elliot Francis

Porphyrinogen via Pd-Carbon

Protocols

CHEMICAL REDUCTION OF PORPHYRINS USING PALLADIUM CARBON Put 5.0mg Pd/C (palladium carbon) and ...

Updated 2 years ago by Elliot Francis

Protoporphyrinogen Oxidase Activity Assay

Protocols

PROTOPORPHYRINOGEN OXIDASE Cultured cell homogenate Resuspend the pellet of PBS-washed cells ...

Updated 2 years ago by Elliot Francis

Pyridine hemochromogen

Protocols

HEME QUANTITATIONS FROM PYRIDINE HEMOCHROMOGEN SPECTRA   Hemochromogen formation   Prepare ...

Updated 2 years ago by Elliot Francis

Reverse FECH

Protocols

Sonicate the washed pellet of cultured bacterial cells in two volumes of 10mM KPi pH 5.5. Deter...

Updated 2 years ago by Elliot Francis

UPLC of Porphyrins

Protocols

ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) QUANTITATION OF PORPHYRINS Inject 10µL of super...

Updated 2 years ago by Elliot Francis

UROD Activity Assay

Protocols

UROPORPHYRINOGEN DEAMINASE (UROD) ASSAY   Reaction A: Uroporphyrinogen (substrate) synthesis  ...

Updated 2 years ago by Elliot Francis

Instrument Types

Cell Imaging User Training Manual

Different microscopes have different uses.   Wide Field Microscope Wide field microscopes illu...

2022

News Publications

July 2021 - June 2022   Batot, G., Metcalf, C., Bell, L., Pauletti, A., Wilcox, K., & Bröer...

2021

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July 2020 - June 2021   Ahmed, S. M. H., Maldera, J. A., Krunic, D., Paiva-Silva, G. O., Pé...

2020

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July 2019 - June 2020   Deshpande, I., Liang, J., Hedeen, D., Roberts, K. J., Zhang, Y., Ha...

2019

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July 2018 - June 2019   Reidy, P. T., McKenzie, A. I., Mahmassani, Z. S., Petrocelli, J. J....

2018

News Publications

July 2017 - June 2018   Abdul-Wajid, S., Demarest, B. L., & Yost, H. J. (2018). Loss of emb...

2023

News Publications

July 2022 - June 2023   Almanzar, D. E., Gordon, S. G., Bristow, C., Hamrick, A., von Diezm...