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If the quote needs to be modified following the drafting and acceptance of the quote, the clients...

Once a quote is accepted during the quote refinement meeting, it is then drafted on the HSC Cores...

The initial quote will undergo a complimentary refinement following the rough draft presentation ...

Next, a meeting will take place between stakeholders to discuss the quote. The quote is either ...

Following the initial meetings with the stakeholders, the SD2C Director will meet with the assign...

An initial meeting will be scheduled between the SD2C and clients/partners/PIs.  This meeting wi...

UROPORPHYRINOGEN III SYNTHASE (U3S) Cytosolic U3S Assay Mix 100 µL 20mM Tris pH 8.2, 260 µL 2...

AMINOLEVULINIC ACID DEHYRATASE (ALAD)   Mouse liver homogenate Weigh out ~100mg liver. Add ...

d-AMINOLEVULINIC ACID SYNTHASE (ALAS) Mouse liver homogenate Weigh out ~100mg liver. Add fou...

BLOOD HEMOGLOBIN ASSAY (DRABKINS)   Drabkins Reagent Dissolve 1.0g NaHCO3, 0.05g KCN and 0.2...

BLOOD UROD   Sample preparation Draw two green-topped collection tubes of blood.  These tube...

COLORIMETRIC PORPHOBILINOGEN ASSAY (EHRLICH) Modified Ehrlich Reagent Dissolve 1.0 gm p-dimet...

COPROPORPHYRINOGEN III OXIDASE (COPOX) ASSAY Reaction A: Uroporphyrinogen III synthesis Add 8...

FERROCHELATASE (FECH) Sample preparation Suspend ~50-µL mammalian cell pellet in 400µL TGD bu...

HEMIN, PROTOPORPHYRIN IX AND ZINC PROTOPORPHYRIN IX EHTYL ACETATE / ACETIC ACID EXTRACTION AND U...

IRON ASSAY USING ICP-OES   Put samples in glass vials. If needed, dry under blowing argon an...

PORPHOBILINOGEN DEAMINASE (PBGD)   PBGD Assay Add 10µL homogenized sample to 75µL in 0.1M Tr...

CHEMICAL REDUCTION OF PORPHYRINS USING PALLADIUM CARBON Put 5.0mg Pd/C (palladium carbon) and ...

PROTOPORPHYRINOGEN OXIDASE Cultured cell homogenate Resuspend the pellet of PBS-washed cells ...

HEME QUANTITATIONS FROM PYRIDINE HEMOCHROMOGEN SPECTRA   Hemochromogen formation   Prepare ...