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An initial meeting will be scheduled between the SD2C and clients/partners/PIs.  This meeting wi...

UROPORPHYRINOGEN III SYNTHASE (U3S) Cytosolic U3S Assay Mix 100 µL 20mM Tris pH 8.2, 260 µL 2...

AMINOLEVULINIC ACID DEHYRATASE (ALAD)   Mouse liver homogenate Weigh out ~100mg liver. Add ...

d-AMINOLEVULINIC ACID SYNTHASE (ALAS) Mouse liver homogenate Weigh out ~100mg liver. Add fou...

BLOOD HEMOGLOBIN ASSAY (DRABKINS)   Drabkins Reagent Dissolve 1.0g NaHCO3, 0.05g KCN and 0.2...

BLOOD UROD   Sample preparation Draw two green-topped collection tubes of blood.  These tube...

COLORIMETRIC PORPHOBILINOGEN ASSAY (EHRLICH) Modified Ehrlich Reagent Dissolve 1.0 gm p-dimet...

COPROPORPHYRINOGEN III OXIDASE (COPOX) ASSAY Reaction A: Uroporphyrinogen III synthesis Add 8...

FERROCHELATASE (FECH) Sample preparation Suspend ~50-µL mammalian cell pellet in 400µL TGD bu...

HEMIN, PROTOPORPHYRIN IX AND ZINC PROTOPORPHYRIN IX EHTYL ACETATE / ACETIC ACID EXTRACTION AND U...

IRON ASSAY USING ICP-OES   Put samples in glass vials. If needed, dry under blowing argon an...

PORPHOBILINOGEN DEAMINASE (PBGD)   PBGD Assay Add 10µL homogenized sample to 75µL in 0.1M Tr...

CHEMICAL REDUCTION OF PORPHYRINS USING PALLADIUM CARBON Put 5.0mg Pd/C (palladium carbon) and ...

PROTOPORPHYRINOGEN OXIDASE Cultured cell homogenate Resuspend the pellet of PBS-washed cells ...

HEME QUANTITATIONS FROM PYRIDINE HEMOCHROMOGEN SPECTRA   Hemochromogen formation   Prepare ...

Sonicate the washed pellet of cultured bacterial cells in two volumes of 10mM KPi pH 5.5. Deter...

ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) QUANTITATION OF PORPHYRINS Inject 10µL of super...

UROPORPHYRINOGEN DEAMINASE (UROD) ASSAY   Reaction A: Uroporphyrinogen (substrate) synthesis  ...

Different microscopes have different uses.   Wide Field Microscope Wide field microscopes illu...

July 2022 - June 2023   Almanzar, D. E., Gordon, S. G., Bristow, C., Hamrick, A., von Diezm...