Zeiss 700 Confocal Microscope

Location: HSC (Building 586) Room 56

The LSM 700 laser scanning confocal microscope is the seventh generation of confocal microscopes from Carl Zeiss. The LSM 700 system uses a Zeiss AXIO Observer Z1 inverted microscope with transmitted illumination (HAL 100) and a laser illumination source. The LSM 700 system features easy operation, excellent sensitivity, and a design that can handle complex tasks.

Main features of Zeiss 700 System:

• Two Photo Multipliers Tube (PMT1 and PMT2) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels.
• Confocal scan head unit can provide high-resolution images up to 2048 x 2048 pixels in size.
• Fully automated Zeiss Observer Z1 inverted microscope.
• LED Epifluorescence and HAL100 Brightfield illumination lamps for rapid sample identification and focusing through the eyepiece.
• Filter wheel used for epifluorescence viewing of the sample is currently outfitted with  DAPI , FITC (green), and Rhodamine (red) filter cube sets.
• Motorized XY stage with a variety of stage inserts available (slides, dishes, multiwall plates, etc..).
• Zeiss Zen Black multi-platform acquisition software.
• Measurements include: Z stack, Time lapse, Stitching/Tiling, XY positions, and Spectra unmixing

Suggested Applications:

• 3D Imaging.

• Multichannel fluorescence fixed slide imaging.
• Tiled mosaics.

Imaging lasers:

Objectives: