Zeiss 880 Airy Scan
Location:CSC Room 032
Main features of Zeiss 880 Airy Scan System:
- Five detectors, Three Photo Multipliers Tube (PMT) detectors and two GaSP detectors for enhanced sensitivity and dynamic range for 5 total fluorescent channels.
- Transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally.
- AIRY scan detector capable of super resolution imaging
- Fast AIRY scan system for high speed super resolution imaging timelapse
- Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
- Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets.
- Laser autofocus system provides focal depth consistency across varying sample regions of interest.
- Piezo Z stage for fast z acquisition
- IBIDI stage top incubator with temperature, C02 and humidity. Plates and dishes with glass bottoms.
Imaging lasers:
- 405nm diode laser
- 488nm Argon gas laser
- 561nm diode
- 638nm diode
Suggested Applications:
- Multichannel fluorescence and transmitted fixed slide imaging
- Tiling mosaics of fixed or live samples
- Timelapse of dishes or glass bottom well plates
Objectives:
|
Objective |
Magnification | Immersion | Numerical Apperature | Correction Ring | Coverglass (mm) | Working Distance (mm) |
| Plan Apo M27 | 5 | Air | 0.16 | 0.17 | 12.1 | |
| EC Plan-Neofluar M27 | 10 | Air | 0.3 | 0.17 | 5.2 | |
| Plan Apo M27 | 20 | Air | 0.8 | 0.17 | 0.55 | |
| Plan Apo M27 | 40 | Oil | 1.3 | 0.17 | 0.21 | |
| Plan Apo DIC M27 | 63 | Oil | 1.4 | 0.17 | 0.19 | |
| Calibration Apo DIC M27 |
No Comments