# Zeiss 700 Confocal Microscope

#### Location: HSC (Building 586) Room 56

[![image-1700073367695.jpeg](https://bookstack.cores.utah.edu/uploads/images/gallery/2023-11/scaled-1680-/image-1700073367695.jpeg)](https://bookstack.cores.utah.edu/uploads/images/gallery/2023-11/image-1700073367695.jpeg)

The LSM 700 laser scanning confocal microscope is the seventh generation of confocal microscopes from Carl Zeiss. The LSM 700 system uses a Zeiss AXIO Observer Z1 inverted microscope with transmitted illumination (HAL 100) and a laser illumination source. The LSM 700 system features easy operation, excellent sensitivity, and a design that can handle complex tasks.

#### **Main features of Zeiss 700 System:**

- Two Photo Multipliers Tube (PMT1 and PMT2) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels.
- Confocal scan head unit can provide high-resolution images up to 2048 x 2048 pixels in size.
- Fully automated Zeiss Observer Z1 inverted microscope.
- LED Epifluorescence and HAL100 Brightfield illumination lamps for rapid sample identification and focusing through the eyepiece.
- Filter wheel used for epifluorescence viewing of the sample is currently outfitted with DAPI , FITC (green), and Rhodamine (red) filter cube sets.
- Motorized XY stage with a variety of stage inserts available (slides, dishes, multiwall plates, etc..).
- Zeiss Zen Black multi-platform acquisition software.
- Measurements include: Z stack, Time lapse, Stitching/Tiling, XY positions, and Spectra unmixing

#### **Suggested Applications:**

- 3D Imaging.
- Multichannel fluorescence fixed slide imaging.
- Tiled mosaics.

#### **Imaging lasers:**

<table border="1" id="bkmrk-wavelength-%28nm%29-type" style="border-collapse: collapse; width: 100%;"><colgroup><col style="width: 24.938272%;"></col><col style="width: 24.938272%;"></col><col style="width: 24.938272%;"></col><col style="width: 24.938272%;"></col></colgroup><tbody><tr><td>Wavelength (nm)</td><td>Type</td><td>Power</td><td>Manufacturer</td></tr><tr><td>405</td><td>diode</td><td>5 mW</td><td width="191">Lasos Lasertechnik GmbH

</td></tr><tr><td>488</td><td>diode</td><td>10 mW</td><td width="191">Lasos Lasertechnik GmbH

</td></tr><tr><td>555</td><td>diode</td><td>10 mW</td><td width="191">Lasos Lasertechnik GmbH

</td></tr><tr><td>639</td><td>diode</td><td>5 mW</td><td width="191">Lasos Lasertechnik GmbH

</td></tr></tbody></table>


#### **Objectives:**

<table border="1" id="bkmrk-magnification-immers" style="border-collapse: collapse; width: 100%; height: 261px;"><colgroup><col style="width: 16.296296%;"></col><col style="width: 13.82716%;"></col><col style="width: 14.814815%;"></col><col style="width: 15.802469%;"></col><col style="width: 13.333333%;"></col><col style="width: 12.716049%;"></col><col style="width: 12.962963%;"></col></colgroup><tbody><tr><td>**Objective**</td><td>**Magnification**</td><td>**Immersion**</td><td>**Numerical**

**Apperature**

</td><td>**Correction Ring**</td><td>**Coverglass (mm)**</td><td>**Working**

**Distance (mm)**

</td></tr><tr><td>EC Plan Neofluar M27</td><td>10X</td><td>Air</td><td>0.3</td><td>  
</td><td>0.17</td><td>5.2

</td></tr><tr><td>Pan Apo M27</td><td>20X</td><td>Air</td><td>0.8</td><td>  
</td><td>0.17</td><td>0.55</td></tr><tr><td>LD C-Apo Korr M27</td><td>40X</td><td>Water</td><td>1.1</td><td>Corr</td><td>0.14-0.19</td><td>0.62</td></tr><tr><td>Pan Apo DIC M27</td><td>63X</td><td>Oil</td><td>1.4</td><td>  
</td><td>0.17</td><td>0.19</td></tr></tbody></table>