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Nikon A1R Confocal

Location: HSC Room 56

Main features of System:

  • Four Photo Multipliers Tube (PMT) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels.
  • Fifth transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally.
  • Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels.
  • Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels.
  • Fully automated Nikon Ti-E inverted microscope.
  • Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
  • Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets.
  • ASI MS-2000 Motorized XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..).
  • MCL Piezo Stage (100um range) for high speed 3D (i.e., Z-stack) volume acquisition.
  • Perfect Focus System provides focal depth consistency across varying sample regions of interest.
  • Nikon NIS-Elements multi-platform acquisition software.

Imaging lasers:

  • 405nm diode laser
  • 488nm Argon gas laser
  • 561nm Sapphire diode laser
  • 638nm diode laser

Suggested Applications:

  • Multichannel fluorescence and transmitted fixed slide imaging
  • Tiling mosaics of fixed or live samples
  • Timelapse of dishes or glass bottom well plates

Objectives:

Objective Magnification Immersion Numerical Aperture Correction Ring Coverglass Working Distance
Plan Apo Lambda 4 Air 0.2

20
Plan Apo Lambda 10 Air 0.45
0.17 4
Plan Apo Lambda 20 Air 0.75
0.17 1
Plan Fluor 40 Oil 1.3
0.17 0.2
Plan Apo Lambda 60 Oil 1.4
0.17 0.13

Other objectives available. Please inquire with Core personnel

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