Leica SP8 405-488-561-633 Laser Confocal

Location: HCI Room 1440

Main features of Leica SP8 White Light System:

• Five detectors, Two Photo Multipliers Tube (PMT) detectors and three HyD detectors for enhanced sensitivity and dynamic range for 5 total fluorescent channels.
• Transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally.
• Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels.
• Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels.
• Fully automated Leica DM8 microscope
• Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
• Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets.
• Laser autofocus system provides focal depth consistency across varying sample regions of interest.
• OkoLab heating/cooling stage top incubator with C02 and humidity. Plates and dishes with glass bottoms.
• z-galvo

Imaging lasers:

• 405nm diode laser
• 488nm Argon gas laser
• White Light continuously tunable pulsed laser 440-700nm
• 561
• 633

Suggested Applications:

• Multichannel fluorescence and transmitted fixed slide imaging
• Tiling mosaics of fixed or live samples
• Timelapse of dishes or glass bottom well plates

Objectives: