Nikon A1R Confocal
Location: HSC Room 56
Main features of System:
- Four Photo Multipliers Tube (PMT) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels.
- Fifth transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally.
- Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels.
- Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels.
- Fully automated Nikon Ti-E inverted microscope.
- Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
- Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets.
- ASI MS-2000 Motorized XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..).
- MCL Piezo Stage (100um range) for high speed 3D (i.e., Z-stack) volume acquisition.
- Perfect Focus System provides focal depth consistency across varying sample regions of interest.
- Nikon NIS-Elements multi-platform acquisition software.
Imaging lasers:
- 405nm diode laser
- 488nm Argon gas laser
- 561nm Sapphire diode laser
- 638nm diode laser
Suggested Applications:
- Multichannel fluorescence and transmitted fixed slide imaging
- Tiling mosaics of fixed or live samples
- Timelapse of dishes or glass bottom well plates
Objectives:
| Objective | Magnification | Immersion | Numerical Aperture | Correction Ring | Coverglass | Working Distance |
| Plan Apo Lambda | 4 | Air | 0.2 | 20 | ||
| Plan Apo Lambda | 10 | Air | 0.45 | 0.17 | 4 | |
| Plan Apo Lambda | 20 | Air | 0.75 | 0.17 | 1 | |
| Plan Fluor | 40 | Oil | 1.3 | 0.17 | 0.2 | |
| Plan Apo Lambda | 60 | Oil | 1.4 | 0.17 | 0.13 |
Other objectives available. Please inquire with Core personnel