# Nikon A1R Confocal

#### Location: HSC Room 56

**Main features of System:**

- Four Photo Multipliers Tube (PMT) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels.
- Fifth transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally.
- Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels.
- Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels.
- Fully automated Nikon Ti-E inverted microscope.
- Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
- Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets.
- ASI MS-2000 Motorized XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..).
- MCL Piezo Stage (100um range) for high speed 3D (i.e., Z-stack) volume acquisition.
- Perfect Focus System provides focal depth consistency across varying sample regions of interest.
- Nikon NIS-Elements multi-platform acquisition software.

**Imaging lasers:**

- 405nm diode laser
- 488nm Argon gas laser
- 561nm Sapphire diode laser
- 638nm diode laser

**Suggested Applications:**

- Multichannel fluorescence and transmitted fixed slide imaging
- Tiling mosaics of fixed or live samples
- Timelapse of dishes or glass bottom well plates

#### Objectives:

<table border="1" id="bkmrk-objective-magnificat" style="border-collapse: collapse; width: 100%;"><colgroup><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col></colgroup><tbody><tr><td>**Objective**</td><td>**Magnification**</td><td>**Immersion**</td><td>**Numerical Aperture**</td><td>**Correction Ring**</td><td>**Coverglass**</td><td>**Working Distance**</td></tr><tr><td>Plan Apo Lambda</td><td>4</td><td>Air</td><td>0.2</td><td>  
</td><td>  
</td><td>20</td></tr><tr><td>Plan Apo Lambda</td><td>10</td><td>Air</td><td>0.45</td><td>  
</td><td>0.17</td><td>4</td></tr><tr><td>Plan Apo Lambda</td><td>20</td><td>Air</td><td>0.75</td><td>  
</td><td>0.17</td><td>1</td></tr><tr><td>Plan Fluor</td><td>40</td><td>Oil</td><td>1.3</td><td>  
</td><td>0.17</td><td>0.2</td></tr><tr><td>Plan Apo Lambda</td><td>60</td><td>Oil</td><td>1.4</td><td>  
</td><td>0.17</td><td>0.13</td></tr></tbody></table>

Other objectives available. Please inquire with Core personnel