# Leica SP8 405-488-561-633 Laser Confocal

Location: HCI Room 1440

**Main features of Leica SP8 White Light System:**

- Five detectors, Two Photo Multipliers Tube (PMT) detectors and three HyD detectors for enhanced sensitivity and dynamic range for 5 total fluorescent channels.
- Transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally.
- Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels.
- Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels.
- Fully automated Leica DM8 microscope
- Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
- Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets.
- Laser autofocus system provides focal depth consistency across varying sample regions of interest.
- OkoLab heating/cooling stage top incubator with C02 and humidity. Plates and dishes with glass bottoms.
- z-galvo

**Imaging lasers:**

- 405nm diode laser
- 488nm Argon gas laser
- White Light continuously tunable pulsed laser 440-700nm
- 561
- 633

**Suggested Applications:**

- Multichannel fluorescence and transmitted fixed slide imaging
- Tiling mosaics of fixed or live samples
- Timelapse of dishes or glass bottom well plates

#### Objectives:

<table border="1" id="bkmrk-objective-magnificat" style="border-collapse: collapse; width: 100%;"><colgroup><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col></colgroup><tbody><tr><td>**Objective**</td><td>**Magnification**</td><td>**Immersion**</td><td>**Numerical Aperture**</td><td>**Correction Collar**</td><td>**Coverslip (mm)**</td><td>**Working Distance (mm)**</td></tr><tr><td>HC PL APO CS2</td><td>10</td><td>Air</td><td>0.4</td><td>  
</td><td>  
</td><td>2.74</td></tr><tr><td>HC PL APO CS2</td><td>20</td><td>Either water, glycerine, or oil</td><td>0.75</td><td>Corr</td><td>  
</td><td>0.66</td></tr><tr><td>HC PL APO CS2</td><td>20</td><td>Air</td><td>0.75</td><td>  
</td><td>0.17</td><td>0.62</td></tr><tr><td>HC PL APO CS2</td><td>40</td><td>Water</td><td>1.1</td><td>Corr</td><td>0.14-0.18</td><td>0.65</td></tr><tr><td>HC PL APO CS2</td><td>40</td><td>Oil</td><td>1.3</td><td>  
</td><td>0.17</td><td>0.24</td></tr><tr><td>HC PL APO CS2</td><td>63</td><td>Oil</td><td>1.4</td><td>  
</td><td>0.17</td><td>0.14</td></tr></tbody></table>