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Blood Hemoglobin Assay (Drabkins)
BLOOD HEMOGLOBIN ASSAY (DRABKINS) Drabkins Reagent Dissolve 1.0g NaHCO3, 0.05g KCN and 0.20g K3Fe(CN)6 in some water. Bring to 1.0L final volume. Store refrigerated. Hemoglobin Assay (formation of cyanomethemoglobin) Pipet 5.0mL Drabkins reagent...
ALAS Activity Assay
d-AMINOLEVULINIC ACID SYNTHASE (ALAS) Mouse liver homogenate Weigh out ~100mg liver. Add four volumes (400µL) of ice-cold 50mM potassium phosphate (KPi) pH7.4. Homogenize with ten up-and-down strokes in a 2-mL glass-Teflon (Potter- Elvehjem) tissue homog...
ALAD Activity Assay
AMINOLEVULINIC ACID DEHYRATASE (ALAD) Mouse liver homogenate Weigh out ~100mg liver. Add 400 µL 1mM DTT (dithiothreitol) in 50mM KPi (potassium phosphate) pH 6.8. Homogenize with ten up-and-down strokes in a 2-mL glass-Teflon (Potter- Elvehjem) tissue...
Sanger Troubleshooting
Meaningful Information in your results folder: The Q20 is the number of bases having a Phred score Q20 which is an error rate of 1 in 100 or accuracy of a base call of 99% Plasmid sequences with good quality will have a Q20 number of 700 and above and ...
Quote Process Flow
Quotes will generally follow the process below: Creation - A quote is generated by the facility and emailed to the recipient. Approval - After creation the quote is email to the recipients for approval Recipients will receive an email with a PDF of the ...
Managing Work Authorizations
Location Manager Interface Link: https://workauth.cores.utah.edu/#/manage Authentication Login using uNID Search Bar The search bar will match any information displayed on the table (eg. Account Title, PI Name, etc.) Modes Pending - Waiting approval f...
Asset Detail Interface
System Fields System fields are automatically managed and cannot be edited directly Asset Tag Number Last Seen - Date this asset was last scanned Location Code - The code of the location the asset is assigned to Organization Code - The code of the or...
Locations Tab
Locations Interface All primary actions in the Utah Web Inventory app start in the Locations Tab. On this tab users will be presented with a a list of active locations into which they have scanned or entered. Locations can be added to this list in 3 ways: ...
Search Tab
The assets search tab replicates the basic functionality of the main website search interface. Users can search by Organization Code to pull up the current status of any organization in the system and have a report on its current inventory status.
Settings Tab
Overview The setting screen is divided in to 8 sections: Scanner - Used to connect to any bound TSL scanners Server Address - Used to specify the target address of the Inventory Server. Should be defaulted to the organization server User Settings - Used ...
Docs Tab
The Docs Tab opens this bookstack in a in-app web browser.
First Steps - Training Procedure
Introduce rules/guidelines. How to access the building, computer, and software; how to book the calendar; follow the SOPs; how to seek help; communication between users. Introduce three portions of the whole imaging system. Bulky hardware could be a pow...