Zeiss 700 Confocal Microscope

Location: HSC (Building 586) Room 56

The LSM 700 laser scanning confocal microscope is the seventh generation of confocal microscopes from Carl Zeiss. The LSM 700 system uses a Zeiss AXIO Observer Z1 inverted microscope with transmitted illumination (HAL 100) and a laser illumination source. The LSM 700 system features easy operation, excellent sensitivity, and a design that can handle complex tasks.

Main features of Zeiss 700 System:

• Two Photo Multipliers Tube (PMT1 and PMT2) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels.
• Confocal scan head unit can provide high-resolution images up to 2048 x 2048 pixels in size.
• Fully automated Zeiss Observer Z1 inverted microscope.
• LED Epifluorescence and HAL100 Brightfield illumination lamps for rapid sample identification and focusing through the eyepiece.
• Filter wheel used for epifluorescence viewing of the sample is currently outfitted with  DAPI , FITC (green), and Rhodamine (red) filter cube sets.
• Motorized XY stage with a variety of stage inserts available (slides, dishes, multiwall plates, etc..).
• Zeiss Zen Black multi-platform acquisition software.
• Measurements include: Z stack, Time lapse, Stitching/Tiling, XY positions, and Spectra unmixing

Suggested Applications:

• 3D Imaging.

• Multichannel fluorescence fixed slide imaging.
• Tiled mosaics.

Imaging lasers:

Wavelength (nm)	Type	Power	Manufacturer
405	diode	5 mW	Lasos Lasertechnik GmbH
488	diode	10 mW	Lasos Lasertechnik GmbH
555	diode	10 mW	Lasos Lasertechnik GmbH
639	diode	5 mW	Lasos Lasertechnik GmbH

Objectives:

Objective	Magnification	Immersion	Numerical Apperature	Correction Ring	Coverglass (mm)	Working Distance (mm)	Link
EC Plan Neofluar M27	10X	Air	0.3		0.17	5.2	Zeiss
Pan Apo M27	20X	Air	0.8		0.17	0.55	Zeiss
LD C-Apo Korr M27	40X	Water	1.1	Corr	0.14-0.19	0.62	Zeiss
Pan Apo DIC M27	63X	Oil	1.4		0.17	0.19	Zeiss