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Zeiss 700 Confocal Microscope

Location: HSC (Building 586) Room 56

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Main features of Zeiss 700 System:

  • Two Photo Multipliers Tube (PMT)PMT1 and PMT2) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels.
  • Confocal scan head unit can provide high-resolution images up to 2048 x 2048 pixels in size.
  • Fully automated Zeiss Observer Z1 inverted microscope.
  • LED Epifluorescence and HAL100 Brightfield illumination lamps for rapid sample identification and focusing.focusing through the eyepiece.
  • Filter wheel used for epifluorescence viewing of the sample is currently outfitted with  DAPI (coming soon), FITC (green), and Rhodamine (red), filter cube sets.
  • Motorized XY stage with a variety of stage inserts available (slides, dishes, multiwall plates, etc..).
  • Zeiss Zen Black multi-platform acquisition software.
  • Measurements include: Z stack, Time lapse, Stitching/Tiling, XY positions, and Spectra unmixing

Suggested Applications:

  • 3D Imaging.

  • Multichannel fluorescence fixed slide imaging.
  • Tiled mosaics.

Imaging lasers:

  • 405nm
    • diodelasersoon)
  • 488nm
    • laser,
  • 555nm
    • laser,
  • 639nm
    • laser,
    Wavelength (comingnm) Type Power Manufacturer
    405diode 5 10mWmW

    Lasos Lasertechnik GmbH

    488diode 10 10mWmW

    Lasos Lasertechnik GmbH

    555diode 10 5mWmW

    Lasos Lasertechnik GmbH

    639diode5 mW

    Lasos Lasertechnik GmbH

      Objectives:

      Objective Magnification Immersion

      Numerical

      Apperature

      		Correction Ring Coverglass (mm)

      Working

      Distance (mm)

      		 Link
      EC Plan Neofluar M2710X Air 0.3
      		 0.17

      5.2

      Zeiss
      Pan Apo M2720X Air 0.8
      		 0.17 0.55 Zeiss
      LD C-Apo Korr M2740X Water 1.1Corr 0.14-0.19 0.62 Zeiss
      Pan Apo DIC M2763X Oil 1.4
      		 0.17 0.19 Zeiss

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