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Zeiss 700 Confocal Microscope

Location: HSC (Building 586) Room 56

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Main features of Zeiss 700 System:

  • Two Photo Multipliers Tube (PMT1 and PMT2) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels.
  • Confocal scan head unit can provide high-resolution images up to 2048 x 2048 pixels in size.
  • Fully automated Zeiss Observer Z1 inverted microscope.
  • LED Epifluorescence and HAL100 Brightfield illumination lamps for rapid sample identification and focusing through the eyepiece.
  • Filter wheel used for epifluorescence viewing of the sample is currently outfitted with  DAPI , FITC (green), and Rhodamine (red) filter cube sets.
  • Motorized XY stage with a variety of stage inserts available (slides, dishes, multiwall plates, etc..).
  • Zeiss Zen Black multi-platform acquisition software.
  • Measurements include: Z stack, Time lapse, Stitching/Tiling, XY positions, and Spectra unmixing

Suggested Applications:

  • 3D Imaging.

  • Multichannel fluorescence fixed slide imaging.
  • Tiled mosaics.

Imaging lasers:

Wavelength (nm) Type Power Manufacturer
405 diode 5 mW

Lasos Lasertechnik GmbH
488 diode 10 mW

Lasos Lasertechnik GmbH
555 diode 10 mW

Lasos Lasertechnik GmbH
639 diode 5 mW

Lasos Lasertechnik GmbH

    Objectives:

    Objective Magnification Immersion

    Numerical

    Apperature

    		 Correction Ring Coverglass (mm)

    Working

    Distance (mm)

    		 Link
    EC Plan Neofluar M27 10X Air 0.3
    		0.17

    5.2

    Zeiss
    Pan Apo M27 20X Air 0.8
    		0.17 0.55 Zeiss
    LD C-Apo Korr M27 40X Water 1.1 Corr 0.14-0.19 0.62 Zeiss
    Pan Apo DIC M27 63X Oil 1.4
    		0.17 0.19 Zeiss

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