Nikon A1R Confocal

 

Location: HSC Room 56

Main features of System:

• Four Photo Multipliers Tube (PMT) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels.
• Fifth transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally.
• Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels.
• Ultra high speed resonant acquisitions up to 420 frames per second at 512 x x 32 pixels.
• Fully automated Nikon Ti-E inverted microscope.
• Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
• Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets.
• ASI MS-2000 Motorized XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..).
• MCL Piezo Stage (100um range) for high speed 3D (i.e., Z-stack) volume acquisition.
• Perfect Focus System provides focal depth consistency across varying sample regions of interest.
• Nikon NIS-Elements multi-platform acquisition software.

Imaging lasers:

• 405nm diode laser
• 488nm Argon gas laser
• 561nm Sapphire diode laser
• 638nm diode laser

Suggested Applications:

• Multichannel fluorescence and transmitted fixed slide imaging
• Tiling mosaics of fixed or live samples
• Timelapse of dishes or glass bottom well plates

Objectives:

Objective	Magnification	Immersion	Numerical Aperture	Correction Ring	Coverglass	Working Distance
Plan Apo Lambda	4	Air	0.2			20
Plan Apo Lambda	10	Air	0.45		0.17	4
Plan Apo Lambda	20	Air	0.75		0.17	1
Plan Fluor	40	Oil	1.3		0.17	0.2
Plan Apo Lambda	60	Oil	1.4		0.17	0.13

Objectives:

• 4X
• 10X
• 20X
• 60X oil
• Other objectives available–pleaseavailable. Please inquire with Core personnel