Leica SP8 405-488-561-633 Laser Confocal
Location: HCI Room 1440
Main features of Leica SP8 White Light System:
- Five detectors, Two Photo Multipliers Tube (PMT) detectors and three HyD detectors for enhanced sensitivity and dynamic range for 5 total fluorescent channels.
- Transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally.
- Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels.
- Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels.
- Fully automated Leica DM8 microscope
- Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
- Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets.
- Laser autofocus system provides focal depth consistency across varying sample regions of interest.
- OkoLab heating/cooling stage top incubator with C02 and humidity. Plates and dishes with glass bottoms.
- z-galvo
Imaging lasers:
- 405nm diode laser
- 488nm Argon gas laser
- White Light continuously tunable pulsed laser 440-700nm
- 561
- 633
Suggested Applications:
- Multichannel fluorescence and transmitted fixed slide imaging
- Tiling mosaics of fixed or live samples
- Timelapse of dishes or glass bottom well plates
Objectives:
| Objective | Magnification | Immersion | Numerical Aperture | Correction Collar | Coverslip (mm) | Working Distance (mm) |
| HC PL APO CS2 | 10 | Air | 0.4 | 2.74 | ||
| HC PL APO CS2 | 20 | Either water, glycerine, or oil | 0.75 | Corr | 0.66 | |
| HC PL APO CS2 | 20 | Air | 0.75 | 0.17 | 0.62 | |
| HC PL APO CS2 | 40 | Water | 1.1 | Corr | 0.14-0.18 | 0.65 |
| HC PL APO CS2 | 40 | Oil | 1.3 | 0.17 | 0.24 | |
| HC PL APO CS2 | 63 | Oil | 1.4 | 0.17 | 0.14 |