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PCR Products Quantitate using a gel (nanodrop isn’t reliable with PCR products) For small templates use 50ng PCR product (please clean it up first as left over primers can mess up sequencing) and 4 picomoles of primer (4uL of a 1uM aliquot) For larger tem...

These are the sequences of the primers that we have aliquots of here in the core for our users to make use of.    M13F(-21)  GTAAAACGACGGCCAGT 3' M13R           CAGGAAACAGCTATGAC 3' T7prom        TAATACGACTCACTATAGG 3' T7term         GCTAGTTATTGCTCAGCGG ...

We currently have three sequencing drop off sites around campus.  HCI - End of the bench 4Y, 4th floor Research North Building. PICKUP TIME 11:00AM Biology Building, Bldg 84, Room 202A (Mailroom in the small fridge).  PICKUP TIME 11:30AM DNA Sequencing Core...

Please submit your samples under the resource system (resource.cores.utah.edu).  When you submit, you will need to have a fasta file for each plasmid ready.  It does not need to contain the inserts, but will need to show the sequence of the backbone (vector). ...

Each project is unique with Next Gen Sequencing. For all projects, please consult with us at no charge. We will work with you to determine the best method to answer your question.  Below is an example of how pricing will vary depending on what the needs of the...

Download from the link on our website (Training/Resources from the DNA Sequencing Page). Unzip the download Install K2 Client first Add the number 155.101.153.88 to the "Server" field when it comes up after install Move the .lic file that downloaded in the...