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IRON ASSAY USING ICP-OES   Put samples in glass vials. If needed, dry under blowing argon and in 60˚C water bath. Add 1mL of a mixture of 5vols of concentrated nitric acid to 2vols concentrated     perchloric acid. Cap loosely and put on a 100˚C heat bl...

HEMIN, PROTOPORPHYRIN IX AND ZINC PROTOPORPHYRIN IX EHTYL ACETATE / ACETIC ACID EXTRACTION AND UPLC   Solvent Extraction Prepare extraction solvent (EA) by mixing four volumes of ethyl acetate to one     volume of glacial acetic acid. Dilute sample homo...

FERROCHELATASE (FECH) Sample preparation Suspend ~50-µL mammalian cell pellet in 400µL TGD buffer (Tris buffered glycerol with dithiothreitol (DTT), made by dissolving 2mL or 2.52 g glycerol and 1.5mg DTT in 8.0 mL 20mM Tris pH 8.0) Sonicate (homogenize) ...

COPROPORPHYRINOGEN III OXIDASE (COPOX) ASSAY Reaction A: Uroporphyrinogen III synthesis Add 8 µL 0.5 µg/µL rPBGD (recombinant porphobilinogen deaminase) stock and 2µL 1µg/µL rU3S (recombinant uroporphyrinogen III synthase) to 70µL    10mM dithiothreitol in...

COLORIMETRIC PORPHOBILINOGEN ASSAY (EHRLICH) Modified Ehrlich Reagent Dissolve 1.0 gm p-dimethyl aminobenzaldehyde in 30mL glacial acetic acid. Add 8.0 mL 70% perchloric acid. Bring to 50 mL with glacial acetic acid. Store in the dark at 4˚C for about 2...

BLOOD UROD   Sample preparation Draw two green-topped collection tubes of blood.  These tubes have heparin as   anticoagulant. Spin down the RBCs (red blood cells) at 2600rpm for 5min at 4˚C. Discard the plasma and buffy coat. Wash RBCs twice by fillin...

BLOOD HEMOGLOBIN ASSAY (DRABKINS)   Drabkins Reagent Dissolve 1.0g NaHCO3, 0.05g KCN and 0.20g K3Fe(CN)6 in some water. Bring to 1.0L final volume. Store refrigerated. Hemoglobin Assay (formation of cyanomethemoglobin) Pipet 5.0mL Drabkins reagent...

d-AMINOLEVULINIC ACID SYNTHASE (ALAS) Mouse liver homogenate Weigh out ~100mg liver. Add four volumes (400µL) of ice-cold 50mM potassium phosphate (KPi) pH7.4. Homogenize with ten up-and-down strokes in a 2-mL glass-Teflon (Potter- Elvehjem) tissue homog...

AMINOLEVULINIC ACID DEHYRATASE (ALAD)   Mouse liver homogenate Weigh out ~100mg liver. Add 400 µL 1mM DTT (dithiothreitol) in 50mM KPi (potassium phosphate) pH 6.8. Homogenize with ten up-and-down strokes in a 2-mL glass-Teflon (Potter- Elvehjem) tissue...

To begin a project, clients/partners/PIs should: 1. First, email the SD2C at sd2c@cores.utah.edu 2. Next, fill out the SD2C Project Request Form via Google Forms.  Note: Make sure to be as detailed as possible when filling out the SD2C Project Google Form. ...

An initial meeting will be scheduled between the SD2C and clients/partners/PIs.  This meeting will include discussions about: 1. Project Objective - overall goal of the project 2. Project Scope - breadth of features to be implemented for this project 3. Po...

Following the initial meetings with the stakeholders, the SD2C Director will meet with the assigned staff to draft a quote.  The SD2C Director and assigned staff member will set a time to draft an initial quote for the project. Here, they will discuss the des...

Next, a meeting will take place between stakeholders to discuss the quote. The quote is either 1. Accepted as-is If the quote is accepted as-is, then it is ready to be drafted by the SD2C Director through the HSC Cores Resource Managment System. 2. Rejecte...

The initial quote will undergo a complimentary refinement following the rough draft presentation to clients/partners/PIs. Any feedback is taken down as notes and relayed in a subsequent refinement quote. 

Once a quote is accepted during the quote refinement meeting, it is then drafted on the HSC Cores Quote Management System.

If the quote needs to be modified following the drafting and acceptance of the quote, the clients/partners/PIs and SD2C Director will discuss modifications to the quote for an extra consulting session fee. See current rate sheet for consulting fees. Modificat...

Once quote is finalized and all parties are satisfied, quote acceptance will occur through parties sent the official quote through the HSC Cores Systems.  Clients/Partners/PIs will be automatically be sent a link to sign from HSC Cores Scheduler and approve o...

Normal operation of the StedyCon microscope through a simple confocal scan.  Separate pages will cover other types of scans, e.g., Z-Stacks, Tiled scans, STED. These documents are to reinforce, not replace, hands on training on the microscope.  All users MUST...

The HSC Cores Work Authorization System is the billing/invoice/quote management system that is unique to HSC Cores. It allows for those internal and external to the University of Utah to be invoiced for Core services. To learn more about how to use the Work Au...

Work Initialization Work will be discussed within the SD2C. It begins with a Design Box Session Work Assignment Lift Brainstorming Lift Creation Lift Assignment Note: This is typical for a new project, but if the SD2C is inheriting a project from a...