Protocols
UROD Activity Assay
UROPORPHYRINOGEN DEAMINASE (UROD) ASSAY Reaction A: Uroporphyrinogen (substrate) synthesis ...
UPLC of Porphyrins
ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) QUANTITATION OF PORPHYRINS Inject 10µL of super...
U3S Activity Assay
UROPORPHYRINOGEN III SYNTHASE (U3S) Cytosolic U3S Assay Mix 100 µL 20mM Tris pH 8.2, 260 µL 2...
Reverse FECH
Sonicate the washed pellet of cultured bacterial cells in two volumes of 10mM KPi pH 5.5. Deter...
Pyridine hemochromogen
HEME QUANTITATIONS FROM PYRIDINE HEMOCHROMOGEN SPECTRA Hemochromogen formation Prepare ...
Protoporphyrinogen Oxidase Activity Assay
PROTOPORPHYRINOGEN OXIDASE Cultured cell homogenate Resuspend the pellet of PBS-washed cells ...
Porphyrinogen via Pd-Carbon
CHEMICAL REDUCTION OF PORPHYRINS USING PALLADIUM CARBON Put 5.0mg Pd/C (palladium carbon) and ...
PBGD Activity Assay
PORPHOBILINOGEN DEAMINASE (PBGD) PBGD Assay Add 10µL homogenized sample to 75µL in 0.1M Tr...
Iron Assay (ICP-OES)
IRON ASSAY USING ICP-OES Put samples in glass vials. If needed, dry under blowing argon an...
Hemin PPIX ZnPPIX Assay
HEMIN, PROTOPORPHYRIN IX AND ZINC PROTOPORPHYRIN IX EHTYL ACETATE / ACETIC ACID EXTRACTION AND U...
FECH Activity Assay
FERROCHELATASE (FECH) Sample preparation Suspend ~50-µL mammalian cell pellet in 400µL TGD bu...
COPOX Activity Assay
COPROPORPHYRINOGEN III OXIDASE (COPOX) ASSAY Reaction A: Uroporphyrinogen III synthesis Add 8...
Colorimetric PBG Assay (Ehrlich)
COLORIMETRIC PORPHOBILINOGEN ASSAY (EHRLICH) Modified Ehrlich Reagent Dissolve 1.0 gm p-dimet...
BLOOD UROD
BLOOD UROD Sample preparation Draw two green-topped collection tubes of blood. These tube...
Blood Hemoglobin Assay (Drabkins)
BLOOD HEMOGLOBIN ASSAY (DRABKINS) Drabkins Reagent Dissolve 1.0g NaHCO3, 0.05g KCN and 0.2...
ALAS Activity Assay
d-AMINOLEVULINIC ACID SYNTHASE (ALAS) Mouse liver homogenate Weigh out ~100mg liver. Add fou...
ALAD Activity Assay
AMINOLEVULINIC ACID DEHYRATASE (ALAD) Mouse liver homogenate Weigh out ~100mg liver. Add ...