Prairie Ultima 2
Location: HSC Room 48B / 48D
Main features of Prairie Ultima 2/Multi-Photon Confocal System:
- Six detectors, four reflected mode Photo Multipliers Tube (PMT) detectors and two transmitted mode PMTs for second harmonic and fluorescence detection.
- High NA water immersion optics including 16x PLANAPO LWD Nikon NA 0.8 and a PLANAPO Correction collar LWD 25x NA 1.1, 60x LUMFL water immersion Olympus NA0.8.
- Resonance scanner for high speed imaging timelapse (~200fps depending on format)
- Piezo Z drive for fast z acquisition, 2 inch travel, XY motorized stage
- Fixed (Z) stage with adjustable (2 inch) travel
- Bioscience Tools stage top incubator with heated pad, heated dish
- Mouse Anesthesia system from VetEquip for in vivo applications (Halothane with scrubber)
- Gas supply, 02, C02, 02/C02 mix
Imaging lasers:
- Coherent Vision II with tuning 705-1060nm pulsed femtosecond range 4000-400mW depending on wavelength
Suggested Applications:
- Deep live cell imaging of zebrafish, mouse in vivo, organ culture and slices, 3D cell culture
- Multichannel fluorescence in vivo, slices or cultures
- Tiling mosaics of fixed or live samples
- Timelapse of whole mount, in vivo mouse, culture
- Second harmonic imaging of collagen or other ECM
Objectives:
48B:
| Objective | Magnification | Immersion | Numerical Aperture | Correction Collar | Coverslip (mm) | Working Distance (mm) |
| Apo | 25 | Water, Water Dipping | 1.1 | Corr | 0-0.17 | 2 |
48D:
| Objective | Magnification | Immersion | Numerical Aperture | Correction Collar | Coverslip (mm) | Working Distance (mm) |
| Plan Apo Lambda | 4 | Air | 0.2 |
|
||
| Plan |
10 | Air | 0.3 | 0.17 |
16 |
|
| UPlanFl | 10 | Air | 0. |
|
||
| LWD | 16 | Water Dipping | 0.8 | 0 |
3 |
|
| Apo | 25 | Water, Water Dipping | 1.1 | Corr | 0-0.17 |
2 |
| Fluor | 40 | Water Dipping | 0.8 | 0 |
2 |