Prairie Ultima 2

Location: HSC Room 48B / 48D

Main features of Prairie Ultima 2/Multi-Photon Confocal System:

• Six detectors, four reflected mode Photo Multipliers Tube (PMT) detectors and two transmitted mode PMTs for second harmonic and fluorescence detection.
• High NA water immersion optics including 16x PLANAPO LWD Nikon NA 0.8 and a PLANAPO Correction collar LWD 25x NA 1.1, 60x LUMFL water immersion Olympus NA0.8.
• Resonance scanner for high speed imaging timelapse (~200fps depending on format)
• Piezo Z drive for fast z acquisition, 2 inch travel, XY motorized stage
• Fixed (Z) stage with adjustable (2 inch) travel
• Bioscience Tools stage top incubator with heated pad, heated dish
• Mouse Anesthesia system from VetEquip for in vivo applications (Halothane with scrubber)
• Gas supply, 02, C02, 02/C02 mix

Imaging lasers:

• Coherent Vision II with tuning 705-1060nm pulsed femtosecond range 4000-400mW depending on wavelength

Suggested Applications:

• Deep live cell imaging of zebrafish, mouse in vivo, organ culture and slices, 3D cell culture
• Multichannel fluorescence in vivo, slices or cultures
• Tiling mosaics of fixed or live samples
• Timelapse of whole mount, in vivo mouse, culture
• Second harmonic imaging of collagen or other ECM

Objectives:

• 10x PLANAPO air NA .3
• 40x W LUMPFL NA 0.75
• 60X W LUMPFL NA 0.8
• 16X LWD NA 0.8
• 25X W LWD Correction collar, NA 1.1