Olympus FV1000 Confocal Microscope
Location: CSC Room 32
Main features of System:
- Three standard Photo Multipliers Tube (PMT) light detectors with two of the channels having tunable spectral bandwidths.
- Simultaneous transmitted light or DIC imaging while scanning confocally.
- Fully automated Olympus IX81 inverted microscope.
- Additional epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
- Motorized Prior II XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..).
- Olympus FV10-ASW 3.1 confocal imaging software
Imaging lasers:
- 405nm diode laser
- 488nm Argon gas laser
- 543nm Helium-neon gas laser
- 633nm Helium-neon gas laser
Objectives:
| Objective | Magnification | Immersion | Numerical Apperature | Correction Ring | Coverglass (mm) | Working Distance (mm) |
| UPlanSApo | 10X | Air | 0.4 | 0.17 | 3.1 | |
| UPlanApo | 20X | Air | 0.7 | 0.17 | 0.65 | |
| UApo | 40X | Oil | 1.35 | Corr | 0.17 | 0.1 |
| PlanApo | 60X | Water | 1.2 | Corr | 0.13-0.21 | 0.28 |
| PlanApo | 60X | Oil | 1.4 | 0.17 | 0.12 |