Nikon Automated Widefield Microscope
Location: HCI Room 1430
Main features of System:
- Widefield light pathway employs a high sensitivity Andor Zyla CMOS camera
- Sola automated LED lightsource for both widefield fluorescence and transmitted illumination pathways
- Fully automated Nikon Ti-E inverted microscope
- Perfect Focus System provides focal depth consistency across varying sample regions of interest
- Motorized Prior II XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..)
- Nikon NIS-Elements multi-platform acquisition software capable of Tiling, timelapse, scripting, analysis and advanced image processing.
- Stage Incubator: OKOLAB, C02 and Humidity with either dish or plate adaptor
Suggested Applications:
- Multichannel fluorescence and transmitted fixed slide imaging
- Tiling mosaics of fixed or live samples
- Timelapse of dishes or glass bottom well plates
Objectives:
| Objective | Magnification | Immersion | Numerical Aperture | Correction Ring | Coverglass (mm) | Working Distance (mm) |
| Plan Apo Lambda | 4 | Air | 0.2 | 20 | ||
| Plan Apo Lambda | 10 | Air | 0.45 |
|
4 | |
| Plan Apo Lambda | 20 | Air | 0.75 | 0.17 | 1 | |
| S PLAN Fluor ADM ELWD | 20 | Air | 0.45 | Corr | 0-2.0 | 8.2-6.9 |
| Plan Apo Lambda | 60 | Oil | 1.4 | 0.17 | 0.13 | |
| Plan Apo Lambda | 100 | Oil | 1.45 | 0.17 | 0.13 |
Other objectives available–pleaseavailable. Please inquire with Core personnel