Nikon Automated Widefield Microscope

Location: HCI Room 1430

Main features of System:

• Widefield light pathway employs a high sensitivity Andor Zyla CMOS camera
• Sola automated LED lightsource for both widefield fluorescence and transmitted illumination pathways
• Fully automated Nikon Ti-E inverted microscope
• Perfect Focus System provides focal depth consistency across varying sample regions of interest
• Motorized Prior II XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..)
• Nikon NIS-Elements multi-platform acquisition software capable of Tiling, timelapse, scripting, analysis and advanced image processing.
• Stage Incubator: OKOLAB, C02 and Humidity with either dish or plate adaptor

Suggested Applications:

• Multichannel fluorescence and transmitted fixed slide imaging
• Tiling mosaics of fixed or live samples
• Timelapse of dishes or glass bottom well plates

Objectives:

Objective	Magnification	Immersion	Numerical Aperture	Correction Ring	Coverglass (mm)	Working Distance (mm)
Plan Apo Lambda	4	Air	0.2			20
Plan Apo Lambda	10	Air	0.45		0.17	4
Plan Apo Lambda	20	Air	0.75		0.17	1
S PLAN Fluor ADM ELWD	20	Air	0.45	Corr	0-2.0	8.2-6.9
Plan Apo Lambda	60	Oil	1.4		0.17	0.13
Plan Apo Lambda	100	Oil	1.45		0.17	0.13

Other objectives available. Please inquire with Core personnel