Nikon Automated Widefield Microscope

Location: HCI Room 1430

Main features of System:

• Widefield light pathway employs a high sensitivity Andor Zyla CMOS camera
• Sola automated LED lightsource for both widefield fluorescence and transmitted illumination pathways
• Fully automated Nikon Ti-E inverted microscope
• Perfect Focus System provides focal depth consistency across varying sample regions of interest
• Motorized Prior II XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..)
• Nikon NIS-Elements multi-platform acquisition software capable of Tiling, timelapse, scripting, analysis and advanced image processing.
• Stage Incubator: OKOLAB, C02 and Humidity with either dish or plate adaptor

Suggested Applications:

• Multichannel fluorescence and transmitted fixed slide imaging
• Tiling mosaics of fixed or live samples
• Timelapse of dishes or glass bottom well plates

Objectives:

• 10X
• 20X
• 40X water
• 40X ELWD
• 60X PlanAPO oil immersion
• Other objectives available–please inquire with Core personnel