Leica Spinning Disk Confocal

Location: EEJMRB Room 5122

Hardware:

• Leica DMi8 motorized microscope with adaptive focus tracking
• ASI motorized stage with Piezo Z
• W-1 large format spinning disk confocal with beam homogenizer
• Andor 1024 x 1024 EMCCD camera
• Visitron 2D FRAP unit
• 405,488,561,640nm laser lines for DAPI, GFP, RFP, Cy5, and similar fluorochromes
• W-View image splitter for dual channel simultaneous imaging
• LED based widefield fluorescence
• Stage top incubator system
• Visiview acquisition software

Primary applications:

• 2D and 3D Multi-Channel Fluorescence Imaging
• 3D Restorative Deconvolution
• Time-Lapse Imaging
• Multipoint Imaging
• Sample images before (A) and after (B) deconvolution