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Untargeted Metabolomics SOP
Standard Operating Procedure - Sample Preparation for Untargeted Metabolomic Analysis 1. Purpose To analyze samples for changes in polar metabolites by liquid-chromatography mass spectrometry. This procedure covers the preparation of extraction solve...
GC/MS Fly Larvae
Standard Operating Procedure - Extraction of Fly Larvae Samples for GC-MS 1. Purpose To extract metabolites from fly larvae for general metabolomics analysis by GC-MS. This procedure covers processing the actual samples, preparation of process blanks...
GC/MS Serum or Plasma
Standard Operating Procedure-Plasma/Serum for GC-MS 1. Purpose To extract metabolites from serum/plasma for general metabolomics analysis by GC-MS. This procedure covers processing the actual samples, preparation of process blanks (PB) samples to det...
GC/MS Tissue
Standard Operating Procedure - Extraction of Liver Tissue Samples for GC-MS 1. Purpose To extract metabolites from liver tissue for general metabolomics analysis by GC-MS. This procedure covers processing the actual samples, preparation of process bl...
GC/MS Sample Preparation
Standard Operating Procedure-Sample Preparation for GC-MS 1. Purpose To prepare samples for GC-MS analysis. This procedure covers the preparation of FAMES (Fatty Acid Methyl Ester) GC reference standard, MOX (Methoxyamine) derivatization of GC sample...
Lipidomics LCMS SOP
Standard Operating Procedure-Sample Preparation for LC-MS 1. Purpose To prepare and analyze samples for LC-MS and/or LC-MS-MS lipidomics. 2. Scope This SOP applies to all LC-MS samples submitted for lipidomic analysis. Samples may come f...
Plasma Lipid Extraction (BuOH:MeOH, Alshehry)
Lipid Ex Alshehry Plasma LCMS v1-1.docx
Tissue Lipid Extraction (BuOH:MeOH, Alshehry)
Lipid Ex Alshehry Tissue LCMS v1-2.docx
Cells Lipid Extraction (BuOH:MeOH, Alshehry)
Lipid Ex Alshehry Cells LCMS v1.docx
Plasma Lipid Extraction (MTBE:MeOH:H2O, Matyash)
Lipid Ex Matyash Plasma v1-1.docx
Tissue Lipid Extraction (MTBE:MeOH:H2O, Matyash)
Lipid Ex Matyash Tissue v1-2.docx
Cells Lipid Extraction (MTBE:MeOH:H2O, Matyash)
Lipid Ex Matyash Cells v1-1.docx