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Search List Available Search Fields ID Username First Name Last Name  User Details ID Username - Should be uNID's where possible Tags - User Groupings System Tags - User Groupings First Name Last Name Email Address Phone Number Department ...

Organization Search Available Fields ID Code Name Organization Details ID Code Name Tags Contact Email Field Configuration Fields that an organization requires appear in this area. These fields come in 5 Types: String - General Text St...

Justification In order to standardize available information and make it easier for existing and potential users to navigate the services available at the University of Utah the Health Science Core Facilities group maintains the main cores website. While indiv...

List Available Printers (Mac OS) lpstat -p -d Print ZPL File (Raw Stream to Printer - Mac OS) lpr -P Zebra_Technologies_ZTC_ZT410R_203dpi_ZPL -o raw <path_to_downloaded_zpl_file> University of Utah ZPL Example Code ^XA ~SD25 ^PON ^LH0,30 ^FO10,30 ^GF...

Fill out and submit a Work Authorization form. Work Auth Form For questions about this process please contact the administrative office. All users must complete the online Laser Safety Training provided by EHS: https://utah.bridgeapp.com/learner/cour...

Work Auth System Overview Prerequisite to gaining access to the cores resource system or being able to request services from any cores managed facility you will be required to fill out a "Work Authorization" form. This form is a digital authorization and data...

UROPORPHYRINOGEN DEAMINASE (UROD) ASSAY   Reaction A: Uroporphyrinogen (substrate) synthesis   Add 10 µL 0.5 µg/µL rPBGD (recombinant porphobilinogen deaminase) stock to   75µL 10mM DTT (dithiothreitol) in 0.1M Tris pH 7.65.  (This is for the           s...

ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) QUANTITATION OF PORPHYRINS Inject 10µL of supernatant solution of porphyrins in 1.5M HCl into a Waters Acquity UPLC system which includes a binary solvent manager, sample manager, fluorescence detector, column ...

UROPORPHYRINOGEN III SYNTHASE (U3S) Cytosolic U3S Assay Mix 100 µL 20mM Tris pH 8.2, 260 µL 20mM KPi (potassium phosphate) pH 8.2 and 20 µL 0.5mg/mL rPBGD (recombinant porphobilinogen      deaminase). Mix well and incubate for at least 2 min at 37˚C. Add...

Sonicate the washed pellet of cultured bacterial cells in two volumes of 10mM KPi pH 5.5. Determine protein content and adjust to about 20mg/mL with the same buffer to prepare the homogenate.  Deoxygenate all samples and reagents. Mix about 50µL homogenate ...

HEME QUANTITATIONS FROM PYRIDINE HEMOCHROMOGEN SPECTRA   Hemochromogen formation   Prepare a pyridine reagent mix by adding 3mL 1M NaOH and 6mL pyridine (use glass pipet) to 19mL H2O in a glass container (28mL total volume). Put 1000µL of the pyridine r...

PROTOPORPHYRINOGEN OXIDASE Cultured cell homogenate Resuspend the pellet of PBS-washed cells in about three volumes of 50mM potassium phosphate pH 7.4 (KPi). Sonicate (homogenize) while in an ice bath at the lowest practicable power setting for 3 cycles x...

CHEMICAL REDUCTION OF PORPHYRINS USING PALLADIUM CARBON Put 5.0mg Pd/C (palladium carbon) and 2.0mg porphyrin in a 13 x 100mm borosilicate test tube.  Put the tube on a suitable tube rack. Wet the contents with 0.2mL water to avoid potential explosion when...

PORPHOBILINOGEN DEAMINASE (PBGD)   PBGD Assay Add 10µL homogenized sample to 75µL in 0.1M Tris pH 7.65. Add 15 µL 2.2mM PBG (porphobilinogen) in 0.1M Tris pH7.65. Incubate in a 37˚C water bath for 30 min. Stop the reaction with 100µL 3M HCl. Expose th...

IRON ASSAY USING ICP-OES   Put samples in glass vials. If needed, dry under blowing argon and in 60˚C water bath. Add 1mL of a mixture of 5vols of concentrated nitric acid to 2vols concentrated     perchloric acid. Cap loosely and put on a 100˚C heat bl...

HEMIN, PROTOPORPHYRIN IX AND ZINC PROTOPORPHYRIN IX EHTYL ACETATE / ACETIC ACID EXTRACTION AND UPLC   Solvent Extraction Prepare extraction solvent (EA) by mixing four volumes of ethyl acetate to one     volume of glacial acetic acid. Dilute sample homo...

FERROCHELATASE (FECH) Sample preparation Suspend ~50-µL mammalian cell pellet in 400µL TGD buffer (Tris buffered glycerol with dithiothreitol (DTT), made by dissolving 2mL or 2.52 g glycerol and 1.5mg DTT in 8.0 mL 20mM Tris pH 8.0) Sonicate (homogenize) ...

COPROPORPHYRINOGEN III OXIDASE (COPOX) ASSAY Reaction A: Uroporphyrinogen III synthesis Add 8 µL 0.5 µg/µL rPBGD (recombinant porphobilinogen deaminase) stock and 2µL 1µg/µL rU3S (recombinant uroporphyrinogen III synthase) to 70µL    10mM dithiothreitol in...

COLORIMETRIC PORPHOBILINOGEN ASSAY (EHRLICH) Modified Ehrlich Reagent Dissolve 1.0 gm p-dimethyl aminobenzaldehyde in 30mL glacial acetic acid. Add 8.0 mL 70% perchloric acid. Bring to 50 mL with glacial acetic acid. Store in the dark at 4˚C for about 2...

BLOOD UROD   Sample preparation Draw two green-topped collection tubes of blood.  These tubes have heparin as   anticoagulant. Spin down the RBCs (red blood cells) at 2600rpm for 5min at 4˚C. Discard the plasma and buffy coat. Wash RBCs twice by fillin...