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HEMIN, PROTOPORPHYRIN IX AND ZINC PROTOPORPHYRIN IX EHTYL ACETATE / ACETIC ACID EXTRACTION AND UPLC   Solvent Extraction Prepare extraction solvent (EA) by mixing four volumes of ethyl acetate to one     volume of glacial acetic acid. Dilute sample homo...

FERROCHELATASE (FECH) Sample preparation Suspend ~50-µL mammalian cell pellet in 400µL TGD buffer (Tris buffered glycerol with dithiothreitol (DTT), made by dissolving 2mL or 2.52 g glycerol and 1.5mg DTT in 8.0 mL 20mM Tris pH 8.0) Sonicate (homogenize) ...

COPROPORPHYRINOGEN III OXIDASE (COPOX) ASSAY Reaction A: Uroporphyrinogen III synthesis Add 8 µL 0.5 µg/µL rPBGD (recombinant porphobilinogen deaminase) stock and 2µL 1µg/µL rU3S (recombinant uroporphyrinogen III synthase) to 70µL    10mM dithiothreitol in...

COLORIMETRIC PORPHOBILINOGEN ASSAY (EHRLICH) Modified Ehrlich Reagent Dissolve 1.0 gm p-dimethyl aminobenzaldehyde in 30mL glacial acetic acid. Add 8.0 mL 70% perchloric acid. Bring to 50 mL with glacial acetic acid. Store in the dark at 4˚C for about 2...

BLOOD UROD   Sample preparation Draw two green-topped collection tubes of blood.  These tubes have heparin as   anticoagulant. Spin down the RBCs (red blood cells) at 2600rpm for 5min at 4˚C. Discard the plasma and buffy coat. Wash RBCs twice by fillin...

BLOOD HEMOGLOBIN ASSAY (DRABKINS)   Drabkins Reagent Dissolve 1.0g NaHCO3, 0.05g KCN and 0.20g K3Fe(CN)6 in some water. Bring to 1.0L final volume. Store refrigerated. Hemoglobin Assay (formation of cyanomethemoglobin) Pipet 5.0mL Drabkins reagent...

d-AMINOLEVULINIC ACID SYNTHASE (ALAS) Mouse liver homogenate Weigh out ~100mg liver. Add four volumes (400µL) of ice-cold 50mM potassium phosphate (KPi) pH7.4. Homogenize with ten up-and-down strokes in a 2-mL glass-Teflon (Potter- Elvehjem) tissue homog...

AMINOLEVULINIC ACID DEHYRATASE (ALAD)   Mouse liver homogenate Weigh out ~100mg liver. Add 400 µL 1mM DTT (dithiothreitol) in 50mM KPi (potassium phosphate) pH 6.8. Homogenize with ten up-and-down strokes in a 2-mL glass-Teflon (Potter- Elvehjem) tissue...

Meaningful Information in your results folder: The Q20 is the number of bases having a Phred score Q20 which is an error rate of 1 in 100 or accuracy of a base call of 99% Plasmid sequences with good quality will have a Q20 number of 700 and above and ...

Quotes will generally follow the process below: Creation - A quote is generated by the facility and emailed to the recipient. Approval - After creation the quote is email to the recipients for approval Recipients will receive an email with a PDF of the ...

Location Manager Interface Link: https://workauth.cores.utah.edu/#/manage Authentication Login using uNID Search Bar The search bar will match any information displayed on the table (eg. Account Title, PI Name, etc.) Modes Pending - Waiting approval f...

System Fields System fields are automatically managed and cannot be edited directly Asset Tag Number  Last Seen - Date this asset was last scanned Location Code - The code of the location the asset is assigned to Organization Code - The code of the or...

Locations Interface All primary actions in the Utah Web Inventory app start in the Locations Tab. On this tab users will be presented with a a list of  active locations into which they have scanned or entered. Locations can be added to this list in 3 ways: ...

The assets search tab replicates the basic functionality of the main website search interface. Users can search by Organization Code to pull up the current status of any organization in the system and have a report on its current inventory status.

Overview The setting screen is divided in to 8 sections: Scanner - Used to connect to any bound TSL scanners Server Address - Used to specify the target address of the Inventory Server. Should be defaulted to the organization server User Settings - Used ...

The Docs Tab opens this bookstack in a in-app web browser.

Introduce rules/guidelines.  How to access the building, computer, and software; how to book the calendar; follow the SOPs; how to seek help; communication between users. Introduce three portions of the whole imaging system.  Bulky hardware could be a pow...