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To create a quote for a facility a user must be listed as a manager on that facility. Users may be listed as managers on multiple facilities. If so care must be taken to select the correct facility that the quote is being created for. To create a quote use the...

Quote States Quotes may be in one of 6 states: Creating - All unsent quotes in the process of being built. Pending - A quote that has been emailed to the user and is awaiting their review and approval/rejection. Approved - A quote that has been accepted ...

External users often have a need for a PDF quote for services prior to starting a project. Using the "Quotes" module managers can create and send PDF quotes to users for approval.  Workflow Managers create a new quote using the Quote Wizard in the Quotes m...

Front Page The front page interface contains quick links for common actions and system overview statistics. Available quick links include: Find Assets - The Main Search Interface Generate Tag - Generate UU branded ZPL RFID Labels (Administrators Only) Re...

Search List Available Search Fields ID Username First Name Last Name  User Details ID Username - Should be uNID's where possible Tags - User Groupings System Tags - User Groupings First Name Last Name Email Address Phone Number Department ...

Organization Search Available Fields ID Code Name Organization Details ID Code Name Tags Contact Email Field Configuration Fields that an organization requires appear in this area. These fields come in 5 Types: String - General Text St...

Justification In order to standardize available information and make it easier for existing and potential users to navigate the services available at the University of Utah the Health Science Core Facilities group maintains the main cores website. While indiv...

List Available Printers (Mac OS) lpstat -p -d Print ZPL File (Raw Stream to Printer - Mac OS) lpr -P Zebra_Technologies_ZTC_ZT410R_203dpi_ZPL -o raw <path_to_downloaded_zpl_file> University of Utah ZPL Example Code ^XA ~SD25 ^PON ^LH0,30 ^FO10,30 ^GF...

Fill out and submit a Work Authorization form. Work Auth Form For questions about this process please contact the administrative office. All users must complete the online Laser Safety Training provided by EHS: https://utah.bridgeapp.com/learner/cour...

Work Auth System Overview Prerequisite to gaining access to the cores resource system or being able to request services from any cores managed facility you will be required to fill out a "Work Authorization" form. This form is a digital authorization and data ...

UROPORPHYRINOGEN DEAMINASE (UROD) ASSAY   Reaction A: Uroporphyrinogen (substrate) synthesis   Add 10 µL 0.5 µg/µL rPBGD (recombinant porphobilinogen deaminase) stock to   75µL 10mM DTT (dithiothreitol) in 0.1M Tris pH 7.65.  (This is for the           s...

ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) QUANTITATION OF PORPHYRINS Inject 10µL of supernatant solution of porphyrins in 1.5M HCl into a Waters Acquity UPLC system which includes a binary solvent manager, sample manager, fluorescence detector, column ...

UROPORPHYRINOGEN III SYNTHASE (U3S) Cytosolic U3S Assay Mix 100 µL 20mM Tris pH 8.2, 260 µL 20mM KPi (potassium phosphate) pH 8.2 and 20 µL 0.5mg/mL rPBGD (recombinant porphobilinogen      deaminase). Mix well and incubate for at least 2 min at 37˚C. Add...

Sonicate the washed pellet of cultured bacterial cells in two volumes of 10mM KPi pH 5.5. Determine protein content and adjust to about 20mg/mL with the same buffer to prepare the homogenate.  Deoxygenate all samples and reagents. Mix about 50µL homogenate ...

HEME QUANTITATIONS FROM PYRIDINE HEMOCHROMOGEN SPECTRA   Hemochromogen formation   Prepare a pyridine reagent mix by adding 3mL 1M NaOH and 6mL pyridine (use glass pipet) to 19mL H2O in a glass container (28mL total volume). Put 1000µL of the pyridine r...

PROTOPORPHYRINOGEN OXIDASE Cultured cell homogenate Resuspend the pellet of PBS-washed cells in about three volumes of 50mM potassium phosphate pH 7.4 (KPi). Sonicate (homogenize) while in an ice bath at the lowest practicable power setting for 3 cycles x...

CHEMICAL REDUCTION OF PORPHYRINS USING PALLADIUM CARBON Put 5.0mg Pd/C (palladium carbon) and 2.0mg porphyrin in a 13 x 100mm borosilicate test tube.  Put the tube on a suitable tube rack. Wet the contents with 0.2mL water to avoid potential explosion when...

PORPHOBILINOGEN DEAMINASE (PBGD)   PBGD Assay Add 10µL homogenized sample to 75µL in 0.1M Tris pH 7.65. Add 15 µL 2.2mM PBG (porphobilinogen) in 0.1M Tris pH7.65. Incubate in a 37˚C water bath for 30 min. Stop the reaction with 100µL 3M HCl. Expose th...