This chapter contains standardized SOPs for lipid extraction used in the Metabolomics Core. Methods include Matyash (MTBE) and Alshehry (BuOH:MeOH) workflows applied to plasma, tissue, and cell samples. These protocols are designed to support both targeted and untargeted LC-MS lipidomics, providing broad lipid class coverage, reproducibility, and compatibility with high-throughput analysis. Users should select the extraction method based on sample type, study design, and downstream analytical goals.