Primer Sequences

These are the sequences of the primers that we have aliquots of here in the core for our users to make use of.  
   
 M13F(-21)   GTAAAACGACGGCCAGT 3' 
 M13R 

 CAGGAAACAGCTATGAC 3' 
 T7prom 

 TAATACGACTCACTATAGG 3' 
 T7term 

 GCTAGTTATTGCTCAGCGG 3' 
 T3prom 

 ATTAACCCTCACTAAAGGG3' 
 SP6 

 GATTTAGGTGACACTATAG 3' 
 CMV-F 

 CGCAAATGGGCGGTAGGCGTG 3' 
 CMV-R 

 AGTAGGAAAGTCCCGTAAGG 
 EGFP-C 

 CATGGTCCTGCTGGAGTTCGTG 
 EGFP-N        CGTCGCCGTCCAGCTCGACCAG 
   
 All users are welcome to come get an aliquot of 50uL of 2uM primer dilutions for use on samples here through our core.  Please do NOT re-freeze the aliquots.  They will store fine in the fridge.  Repeated freeze/thaw cycles of unconcentrated primers can be problematic is Sanger sequencing.