Pyridine hemochromogen

HEME QUANTITATIONS FROM PYRIDINE HEMOCHROMOGEN SPECTRA

 

Hemochromogen formation

 

Calculations

Reference Information

From: Berry, E.A. and Trumpower, B.L. 1987. Simultaneous determination of hemes a, b, and c from pyridine hemochrome spectra.  Analytical Biochemistry161: 1-15.

Table 4

Inverse matrix of extinction coefficients of pyridine hemochromes for calculating concentration (mM) from reduced minus oxidized absorbance at five different wavelengths.

Wavelength (nm):    540                 549                 558                 588                 620  

Heme c:               -0.02778        0.04757       -0.01906         0.00084       -0.00157

Heme b:               -0.02943       -0.00088         0.04037        -0.00267       -0.00738

Heme a:               -0.02687        0.00456         0.00699          0.04353       -0.02820

Total heme may also be obtained by adding the results for hemes a, b and c for a sample.  Authentic heme solutions with known concentrations may be used to check the method and also to help check that the calculations were performed correctly to obtain expected results.


Revision #2
Created 1 December 2023 21:54:16 by Elliot Francis
Updated 4 December 2023 16:14:32 by Elliot Francis