# Prairie Ultima 2

Location: HSC Room 48B / 48D

**Main features of Prairie Ultima 2/Multi-Photon Confocal System:**

- Six detectors, four reflected mode Photo Multipliers Tube (PMT) detectors and two transmitted mode PMTs for second harmonic and fluorescence detection.
- High NA water immersion optics including 16x PLANAPO LWD Nikon NA 0.8 and a PLANAPO Correction collar LWD 25x NA 1.1, 60x LUMFL water immersion Olympus NA0.8.
- Resonance scanner for high speed imaging timelapse (~200fps depending on format)
- Piezo Z drive for fast z acquisition, 2 inch travel, XY motorized stage
- Fixed (Z) stage with adjustable (2 inch) travel
- Bioscience Tools stage top incubator with heated pad, heated dish
- Mouse Anesthesia system from VetEquip for in vivo applications (Halothane with scrubber)
- Gas supply, 02, C02, 02/C02 mix

**Imaging lasers:**

- Coherent Vision II with tuning 705-1060nm pulsed femtosecond range 4000-400mW depending on wavelength

**Suggested Applications:**

- Deep live cell imaging of zebrafish, mouse in vivo, organ culture and slices, 3D cell culture
- Multichannel fluorescence in vivo, slices or cultures
- Tiling mosaics of fixed or live samples
- Timelapse of whole mount, in vivo mouse, culture
- Second harmonic imaging of collagen or other ECM

#### Objectives:

##### 48B:

<table border="1" id="bkmrk-objective-magnificat" style="border-collapse: collapse; width: 100%;"><colgroup><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col></colgroup><tbody><tr><td>**Objective**</td><td>**Magnification**</td><td>**Immersion**</td><td>**Numerical Aperture**</td><td>**Correction Collar**</td><td>**Coverslip (mm)**</td><td>**Working Distance (mm)**</td></tr><tr><td>Apo</td><td>25</td><td>Water, Water Dipping</td><td>1.1</td><td>Corr</td><td>0-0.17</td><td>2</td></tr></tbody></table>

##### 48D:

<table border="1" id="bkmrk-objective-magnificat-1" style="border-collapse: collapse; width: 100%;"><colgroup><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col></colgroup><tbody><tr><td>**Objective**</td><td>**Magnification**</td><td>**Immersion**</td><td>**Numerical Aperture**</td><td>**Correction Collar**</td><td>**Coverslip (mm)**</td><td>**Working Distance (mm)**</td></tr><tr><td>Plan Apo Lambda</td><td>4</td><td>Air</td><td>0.2</td><td>  
</td><td>  
</td><td>20

</td></tr><tr><td>Plan Fluor</td><td>10</td><td>Air</td><td>0.3</td><td>  
</td><td>0.17</td><td>16

</td></tr><tr><td>UPlanFl</td><td>10</td><td>Air</td><td>0.3</td><td>  
</td><td>  
</td><td></td></tr><tr><td>LWD</td><td>16</td><td>Water Dipping</td><td>0.8</td><td>  
</td><td>0</td><td>3

</td></tr><tr><td>Apo</td><td>25</td><td>Water, Water Dipping</td><td>1.1</td><td>Corr</td><td>0-0.17</td><td>2

</td></tr><tr><td>Fluor</td><td>40</td><td>Water Dipping</td><td>0.8</td><td>  
</td><td>0</td><td>2

</td></tr></tbody></table>