# Olympus FV1000 Confocal Microscope

Location: CSC Room 32

**Main features of System:**

- Three standard Photo Multipliers Tube (PMT) light detectors with two of the channels having tunable spectral bandwidths.
- Simultaneous transmitted light or DIC imaging while scanning confocally.
- Fully automated Olympus IX81 inverted microscope.
- Additional epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing.
- Motorized Prior II XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..).
- Olympus FV10-ASW 3.1 confocal imaging software

**Imaging lasers:**

- 405nm diode laser
- 488nm Argon gas laser
- 543nm Helium-neon gas laser
- 633nm Helium-neon gas laser

**Objectives:**

<table border="1" id="bkmrk-objective-magnificat" style="border-collapse: collapse; width: 100%;"><colgroup><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col></colgroup><tbody><tr><td>**Objective**</td><td>**Magnification**</td><td>**Immersion** </td><td>**Numerical Apperature**</td><td>**Correction Ring**</td><td>**Coverglass (mm)**</td><td>**Working Distance (mm)**</td></tr><tr><td>UPlanSApo</td><td>10X</td><td>Air</td><td>0.4</td><td>  
</td><td>0.17</td><td>3.1</td></tr><tr><td>UPlanApo</td><td>20X</td><td>Air</td><td>0.7</td><td>  
</td><td>0.17</td><td>0.65</td></tr><tr><td>UApo</td><td>40X</td><td>Oil</td><td>1.35</td><td>Corr</td><td>0.17</td><td>0.1</td></tr><tr><td>PlanApo</td><td>60X</td><td>Water</td><td>1.2</td><td>Corr</td><td>0.13-0.21</td><td>0.28</td></tr><tr><td>PlanApo</td><td>60X</td><td>Oil</td><td>1.4</td><td>  
</td><td>0.17</td><td>0.12</td></tr></tbody></table>