# Nikon Automated Widefield Microscope

Location: HCI Room 1430

**Main features of System:**

- Widefield light pathway employs a high sensitivity Andor Zyla CMOS camera
- Sola automated LED lightsource for both widefield fluorescence and transmitted illumination pathways
- Fully automated Nikon Ti-E inverted microscope
- Perfect Focus System provides focal depth consistency across varying sample regions of interest
- Motorized Prior II XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..)
- Nikon NIS-Elements multi-platform acquisition software capable of Tiling, timelapse, scripting, analysis and advanced image processing.
- Stage Incubator: OKOLAB, C02 and Humidity with either dish or plate adaptor

**Suggested Applications:**

- Multichannel fluorescence and transmitted fixed slide imaging
- Tiling mosaics of fixed or live samples
- Timelapse of dishes or glass bottom well plates

#### Objectives:

<table border="1" id="bkmrk-objective-magnificat" style="border-collapse: collapse; width: 100%;"><colgroup><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col></colgroup><tbody><tr><td>**Objective**</td><td>**Magnification**</td><td>**Immersion**</td><td>**Numerical Aperture**</td><td>**Correction Ring**</td><td>**Coverglass (mm)**</td><td>**Working Distance (mm)**</td></tr><tr><td>Plan Apo Lambda</td><td>4</td><td>Air</td><td>0.2</td><td>  
</td><td>  
</td><td>20</td></tr><tr><td>Plan Apo Lambda</td><td>10</td><td>Air</td><td>0.45</td><td>  
</td><td>0.17

</td><td>4</td></tr><tr><td>Plan Apo Lambda</td><td>20</td><td>Air</td><td>0.75</td><td>  
</td><td>0.17</td><td>1</td></tr><tr><td>S PLAN Fluor ADM ELWD</td><td>20</td><td>Air</td><td>0.45</td><td>Corr</td><td>0-2.0</td><td>8.2-6.9</td></tr><tr><td>Plan Apo Lambda</td><td>60</td><td>Oil</td><td>1.4</td><td>  
</td><td>0.17</td><td>0.13</td></tr><tr><td>Plan Apo Lambda</td><td>100</td><td>Oil</td><td>1.45</td><td>  
</td><td>0.17</td><td>0.13</td></tr></tbody></table>

Other objectives available. Please inquire with Core personnel