# Leica Spinning Disk Confocal

Location: EEJMRB Room 5122

**Hardware:**

- Leica DMi8 motorized microscope with adaptive focus tracking
- ASI motorized stage with Piezo Z
- W-1 large format spinning disk confocal with beam homogenizer
- Andor 1024 x 1024 EMCCD camera
- Visitron 2D FRAP unit
- 405,488,561,640nm laser lines for DAPI, GFP, RFP, Cy5, and similar fluorochromes
- W-View image splitter for dual channel simultaneous imaging
- LED based widefield fluorescence
- Stage top incubator system
- Visiview acquisition software

**Primary applications:**

- 2D and 3D Multi-Channel Fluorescence Imaging
- 3D Restorative Deconvolution
- Time-Lapse Imaging
- Multipoint Imaging
- Sample images before (A) and after (B) deconvolution

#### Objectives:

<table border="1" id="bkmrk-objective-magnificat" style="border-collapse: collapse; width: 100%;"><colgroup><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col><col style="width: 14.320988%;"></col></colgroup><tbody><tr><td>**Objective**</td><td>**Magnification**</td><td>**Immersion**</td><td>**Numerical Aperture**</td><td>**Correction Ring**</td><td>**Coverglass (mm)**</td><td>**Working Distance (mm)**</td></tr><tr><td>HC PL APO CS2</td><td>10</td><td>Air</td><td>0.4</td><td>  
</td><td>  
</td><td>2.74</td></tr><tr><td>HCX PL APO CS</td><td>20</td><td>Air</td><td>0.75</td><td>  
</td><td>0.17</td><td>0.62</td></tr><tr><td>HC PL APO CS2</td><td>40</td><td>Water</td><td>1.1</td><td>Corr</td><td>0.14-0.18</td><td>0.65</td></tr><tr><td>HC PL APO CS2</td><td>63</td><td>Oil</td><td>1.4</td><td>  
</td><td>0.17</td><td>0.14</td></tr></tbody></table>