Evos Auto Color and Fluor Microscope

Location: HSC (Building 586) Room 54

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The EVOS FL Imaging system is an all-in-one fluorescence microscope designed for efficiency and ease of use. The on-board computer and integrated imaging software allows users to capture and save four channels fluorescence images/data directly from the microscope. The EVOS FL Imaging System is suitable for checking samples before using a high-end microscope system.

Main features of System:

Suggested Applications:

Light Sources:

LED light cubes which combine bright LED illumination with excitation and emission filters.  DAPI, CY5, GFP, RFP and Texas Red cubes are available.  Four can be installed at one time.  Contact Cell Imaging staff to change cubes if needed.  A selection guide for matching cubes to dyes is on the ThermoFisher site: Guide

Cube Excitation (nm) Emission (nm)
DAPI 357/44 447/60
GFP 485/25 524/24
RFP 531/40 593/40
Texas Red 585/29 628/32
Cy5 628/40 692/40

Objectives:

The objectives on the Evos are Long Working Distance, not Coverslip-Corrected.  They are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm.

Objective Magnification Immersion

Numerical

Apperature

Correction Ring Coverglass (mm)

Working

Distance (mm)

PlanApo 1.25X Air 0.04

5.10

Plan Fluor 4X Air 0.13

17.2

Plan Fluor 10X Air 0.3
1.2 8.31
Plan Fluor 20X Air 0.45
1.2 7.11
Plan Fluor 40X Air 0.65
1.2 2.81



Revision #8
Created 20 November 2023 21:27:06
Updated 20 March 2024 19:28:28