Instrument Details Details about each instrument Zeiss 700 Confocal Microscope Location: HSC (Building 586) Room 56 The LSM 700 laser scanning confocal microscope is the seventh generation of confocal microscopes from Carl Zeiss. The LSM 700 system uses a Zeiss AXIO Observer Z1 inverted microscope with transmitted illumination (HAL 100) and a laser illumination source. The LSM 700 system features easy operation, excellent sensitivity, and a design that can handle complex tasks. Main features of Zeiss 700 System: Two Photo Multipliers Tube (PMT1 and PMT2) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels. Confocal scan head unit can provide high-resolution images up to 2048 x 2048 pixels in size. Fully automated Zeiss Observer Z1 inverted microscope. LED Epifluorescence and HAL100 Brightfield illumination lamps for rapid sample identification and focusing through the eyepiece. Filter wheel used for epifluorescence viewing of the sample is currently outfitted with  DAPI , FITC (green), and Rhodamine (red) filter cube sets. Motorized XY stage with a variety of stage inserts available (slides, dishes, multiwall plates, etc..). Zeiss Zen Black multi-platform acquisition software. Measurements include: Z stack, Time lapse, Stitching/Tiling, XY positions, and Spectra unmixing Suggested Applications: 3D Imaging. Multichannel fluorescence fixed slide imaging. Tiled mosaics. Imaging lasers: Wavelength (nm) Type Power Manufacturer 405 diode 5 mW Lasos Lasertechnik GmbH 488 diode 10 mW Lasos Lasertechnik GmbH 555 diode 10 mW Lasos Lasertechnik GmbH 639 diode 5 mW Lasos Lasertechnik GmbH Objectives: Objective Magnification Immersion Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm) EC Plan Neofluar M27 10X Air 0.3 0.17 5.2 Pan Apo M27 20X Air 0.8 0.17 0.55 LD C-Apo Korr M27 40X Water 1.1 Corr 0.14-0.19 0.62 Pan Apo DIC M27 63X Oil 1.4 0.17 0.19 Prairie Ultima 2 Location: HSC Room 48B / 48D Main features of Prairie Ultima 2/Multi-Photon Confocal System: Six detectors, four reflected mode Photo Multipliers Tube (PMT) detectors and two transmitted mode PMTs for second harmonic and fluorescence detection. High NA water immersion optics including 16x PLANAPO LWD Nikon NA 0.8 and a PLANAPO Correction collar LWD 25x NA 1.1, 60x LUMFL water immersion Olympus NA0.8. Resonance scanner for high speed imaging timelapse (~200fps depending on format) Piezo Z drive for fast z acquisition, 2 inch travel, XY motorized stage Fixed (Z) stage with adjustable (2 inch) travel Bioscience Tools stage top incubator with heated pad, heated dish Mouse Anesthesia system from VetEquip for in vivo applications (Halothane with scrubber) Gas supply, 02, C02, 02/C02 mix Imaging lasers: Coherent Vision II with tuning 705-1060nm pulsed femtosecond range 4000-400mW depending on wavelength Suggested Applications: Deep live cell imaging of zebrafish, mouse in vivo, organ culture and slices, 3D cell culture Multichannel fluorescence in vivo, slices or cultures Tiling mosaics of fixed or live samples Timelapse of whole mount, in vivo mouse, culture Second harmonic imaging of collagen or other ECM Objectives: 48B: Objective Magnification Immersion Numerical Aperture Correction Collar Coverslip (mm) Working Distance (mm) Apo 25 Water, Water Dipping 1.1 Corr 0-0.17 2 48D: Objective Magnification Immersion Numerical Aperture Correction Collar Coverslip (mm) Working Distance (mm) Plan Apo Lambda 4 Air 0.2 20 Plan Fluor 10 Air 0.3 0.17 16 UPlanFl 10 Air 0.3 LWD 16 Water Dipping 0.8 0 3 Apo 25 Water, Water Dipping 1.1 Corr 0-0.17 2 Fluor 40 Water Dipping 0.8 0 2 Nikon A1R Confocal Location: HSC Room 56 Main features of System: Four Photo Multipliers Tube (PMT) detector unit enables simultaneous 12 bit (4096 intensity levels) acquisition of up to 4 fluorescent channels. Fifth transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally. Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels. Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels. Fully automated Nikon Ti-E inverted microscope. Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing. Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets. ASI MS-2000 Motorized XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..). MCL Piezo Stage (100um range) for high speed 3D (i.e., Z-stack) volume acquisition. Perfect Focus System provides focal depth consistency across varying sample regions of interest. Nikon NIS-Elements multi-platform acquisition software. Imaging lasers: 405nm diode laser 488nm Argon gas laser 561nm Sapphire diode laser 638nm diode laser Suggested Applications: Multichannel fluorescence and transmitted fixed slide imaging Tiling mosaics of fixed or live samples Timelapse of dishes or glass bottom well plates Objectives: Objective Magnification Immersion Numerical Aperture Correction Ring Coverglass Working Distance Plan Apo Lambda 4 Air 0.2 20 Plan Apo Lambda 10 Air 0.45 0.17 4 Plan Apo Lambda 20 Air 0.75 0.17 1 Plan Fluor 40 Oil 1.3 0.17 0.2 Plan Apo Lambda 60 Oil 1.4 0.17 0.13 Other objectives available. Please inquire with Core personnel Evos Auto Color and Fluor Microscope Location: HSC (Building 586) Room 54 The EVOS FL Imaging system is an all-in-one fluorescence microscope designed for efficiency and ease of use. The on-board computer and integrated imaging software allows users to capture and save four channels fluorescence images/data directly from the microscope. The EVOS FL Imaging System is suitable for checking samples before using a high-end microscope system. Main features of System: Dual 16-bit CCD acquisition cameras (Monochromatic and Color) 22” high-resolution touch screen color monitor Automated X-Y scanning stage with interchangable specimen holder inserts.  We have available inserts for glass slides, culture dishes, multiwell plates, and T-flasks EVOS LED Light Cube Technology.  We have following cubes available: DAPI, GFP, RFP, Texas Red, and Cy5 Suggested Applications: Fixed and live cell imaging. Tissue imaging. Sample check before using a high-end microscope system. Light Sources: LED light cubes which combine bright LED illumination with excitation and emission filters.  DAPI, CY5, GFP, RFP and Texas Red cubes are available.  Four can be installed at one time.  Contact Cell Imaging staff to change cubes if needed.  A selection guide for matching cubes to dyes is on the ThermoFisher site: Guide Cube Excitation (nm) Emission (nm) DAPI 357/44 447/60 GFP 485/25 524/24 RFP 531/40 593/40 Texas Red 585/29 628/32 Cy5 628/40 692/40 Objectives: The objectives on the Evos are Long Working Distance, not Coverslip-Corrected.  They are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. Objective Magnification Immersion Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm) PlanApo 1.25X Air 0.04 5.10 Plan Fluor 4X Air 0.13 17.2 Plan Fluor 10X Air 0.3 1.2 8.31 Plan Fluor 20X Air 0.45 1.2 7.11 Plan Fluor 40X Air 0.65 1.2 2.81 Zeiss Axio Scan.Z1 Location: HSC (Building 586) Room 60 The Zeiss Axio-Scan.Z1 is a fast and flexible slide scanner that digitizes specimens to produce high-quality virtual slides. In addition to brightfield slide scanning, the Axio-Scan.Z1 uses LED lights to scan up to nine fluorescent channels. Up to 100 slides can be loaded onto the scanner, making it suitable for large projects, including digital archives. Scanned images are saved as CZI files and can be viewed using Zeiss freeware Zen-lite or third-party image analysis programs. Main features of Axio Scan.Z1: Holds up to 100 slides. Able to image both bright-field (e.g. H&E slides) and up to nine-colour fluorescent samples.  Image areas can be detected automatically or marked out, to form montages of the whole sample, or multiple samples on each slide Setup wizard to guide users through optimising image acquisition and autofocus parameters. Once setup, the system can be left for unattended automated scanning e.g. overnight Light Sources: In addition to an internal lamp for brightfield imaging, the Axio Scan.Z1 has an X-Cite 120LED source for fluorescent imaging. Filter cubes for LEDs: The scanner has a 10 position filter turret to fit most fluorophores.  The Zen profile wizard is used to pick filter to match your imaging needs.  Alternate filter cubes can be installed for specialty applications.  Please consult with Cell Imaging staff for consultation and assistance. Filter Position Excitation (nm) Emission (nm) Typical Fluorophores Opal Dye 1 empty empty Brightfield 2 325-375 435-485 DAPI 3 426-446 465-495 Cyan, Protein 480 4 480-500 510-530 Alexa Fluor 488 520 5 510-530 540-560 Alexa Fluor 489 540 6 538-562 570-640 HE, DsRed, Alexa Fluor 555 570 7 568-592 610-640 Alexa Fluor 568 620 8 610-630 645-675 Alexa Fluor 660 650 9 625-655 666-715 Cy5 10 650-680 695-755 Alexa Fluor 680 690 Objectives: Objective Magnification Immersion Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm) EC Plan-Neofluar M27 2.5X Air 0.085 0.17 8.8 Fluor M27 2.5X Air 0.12 0.17 8.7 Fluar M27 5X Air 0.25 0.17 12.5 Plan Apo M27 10X Air 0.45 0.17 2.1 Plan Apo M27 20X Air 0.8 0.17 0.55 Plan Apo Korr M27 40X Air 0.95 0.13-0.21 0.25     Nikon Automated Widefield Microscope Location: HCI Room 1430 Main features of System: Widefield light pathway employs a high sensitivity Andor Zyla CMOS camera Sola automated LED lightsource for both widefield fluorescence and transmitted illumination pathways Fully automated Nikon Ti-E inverted microscope Perfect Focus System provides focal depth consistency across varying sample regions of interest Motorized Prior II XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..) Nikon NIS-Elements multi-platform acquisition software capable of Tiling, timelapse, scripting, analysis and advanced image processing. Stage Incubator: OKOLAB, C02 and Humidity with either dish or plate adaptor Suggested Applications: Multichannel fluorescence and transmitted fixed slide imaging Tiling mosaics of fixed or live samples Timelapse of dishes or glass bottom well plates Objectives: Objective Magnification Immersion Numerical Aperture Correction Ring Coverglass (mm) Working Distance (mm) Plan Apo Lambda 4 Air 0.2 20 Plan Apo Lambda 10 Air 0.45 0.17 4 Plan Apo Lambda 20 Air 0.75 0.17 1 S PLAN Fluor ADM ELWD 20 Air 0.45 Corr 0-2.0 8.2-6.9 Plan Apo Lambda 60 Oil 1.4 0.17 0.13 Plan Apo Lambda 100 Oil 1.45 0.17 0.13 Other objectives available. Please inquire with Core personnel Leica SP8 White Light System Location: HCI Room 1480 Main features of Leica SP8 White Light System: Five detectors, Two Photo Multipliers Tube (PMT) detectors and three HyD detectors for enhanced sensitivity and dynamic range for 5 total fluorescent channels. Transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally. Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels. Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels. Fully automated Leica DM8 microscope Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing. Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets. Laser autofocus system provides focal depth consistency across varying sample regions of interest. OkoLab heating/cooling stage top incubator with C02 and humidity. Plates and dishes with glass bottoms. Imaging lasers: 405nm diode laser 488nm Argon gas laser White Light continuously tunable pulsed laser 440-700nm Suggested Applications: Multichannel fluorescence and transmitted fixed slide imaging Tiling mosaics of fixed or live samples Timelapse of dishes or glass bottom well plates Objectives: Objective Magnification Immersion Numerical Aperture Correction Ring Coverglass (mm) Working Distance (mm) HC PL APO CS2 10 Air 0.4 2.74 HC PL APO CS2 20 Either water, glycerine, or oil 0.75 Corr 0.66 HC PL APO CS2 20 Air 0.75 0.17 0.62 HC PL APO CS2 40 Water 1.1 Corr 0.14-0.18 0.65 HC PL APO CS2 40 Oil 1.3 0.17 0.24 HC PL APO CS2 60 Oil 1.4 0.17 0.14 Other objectives available. Please inquire with Core personnel Leica SP8 405-488-561-633 Laser Confocal Location: HCI Room 1440 Main features of Leica SP8 White Light System: Five detectors, Two Photo Multipliers Tube (PMT) detectors and three HyD detectors for enhanced sensitivity and dynamic range for 5 total fluorescent channels. Transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally. Hybrid confocal scan head unit can provide high resolution images up to 4096 x 4096 pixels in size in conventional galvonometer mode and in high speed resonant mode up to 30 frames per second at 512 x 512 pixels. Ultra high speed resonant acquisitions up to 420 frames per second at 512 x 32 pixels. Fully automated Leica DM8 microscope Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing. Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets. Laser autofocus system provides focal depth consistency across varying sample regions of interest. OkoLab heating/cooling stage top incubator with C02 and humidity. Plates and dishes with glass bottoms. z-galvo Imaging lasers: 405nm diode laser 488nm Argon gas laser White Light continuously tunable pulsed laser 440-700nm 561 633 Suggested Applications: Multichannel fluorescence and transmitted fixed slide imaging Tiling mosaics of fixed or live samples Timelapse of dishes or glass bottom well plates Objectives: Objective Magnification Immersion Numerical Aperture Correction Collar Coverslip (mm) Working Distance (mm) HC PL APO CS2 10 Air 0.4 2.74 HC PL APO CS2 20 Either water, glycerine, or oil 0.75 Corr 0.66 HC PL APO CS2 20 Air 0.75 0.17 0.62 HC PL APO CS2 40 Water 1.1 Corr 0.14-0.18 0.65 HC PL APO CS2 40 Oil 1.3 0.17 0.24 HC PL APO CS2 63 Oil 1.4 0.17 0.14 Zeiss Axioscan 7 Location: HCI Room 1470 The Zeiss AxioScan 7 is a fast and flexible slide scanner that digitizes specimens to produce high-quality virtual slides. In addition to brightfield slide scanning, the Axioscan 7 can scan up to five fluorescent channels using fast switching LED lights. Up to 100 slides can be loaded onto the scanner, making it suitable for large projects, including digital archives. Scanned images are saved as CZI files and can be viewed using Zeiss freeware Zen-lite or third-party image analysis programs. Main Features of the Axioscan 7: Holds up to 100 slides. Able to image both bright-field (e.g. H&E slides) and up to nine-colour fluorescent samples.  Image areas can be detected automatically or marked out, to form montages of the whole sample, or multiple samples on each slide Setup wizard to guide users through optimising image acquisition and autofocus parameters. Once setup, the system can be left for unattended automated scanning e.g. overnight Suggested Applications: Fixed slides. Configurations: The Axioscan 7 has a replaceable filter turret and can be switched between two light sources.  The 10 position filter cube turret is typically used with the Colibri 7 switchable LED source.  The fast filter wheel is typically used with the X-Cite Xylis LED source. Configuration Light Source Filter Set 1 Colibri 7 Filter Cubes 2 X-Cite Xylis Fast Filter Wheel Only Cell Imaging staff are permitted to switch between configurations.   Please schedule our time and plan accordingly.   Light Sources: Colibri 7: The Colibri 7 has 6 LEDs with excitation filters to provide 7 excitation bands.  (One LED has a motorized filter to split it into two bands.) Excitation Bands (There is one too many here) Band Wavelength / Bandwidth (nm) Some typical fluorophores UV 385/30 API, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue V 423/44 Pacific Blue, Lucifer Yellow, Alexa Fluor 433, eCFP, Cerulean B 469/38 FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC / Fluorescein, Kaede (green / red), PerCP, YoYo-1,FuraRed C 511/44 Rhodamine 123, Fluo-4, Oregon Green BAPTA, Sytox Green, eYFP, FM4-64, Eosin/HE, Acridine Orange, JC1, Bodipy FL, Propidium Iodide, Spectrum Green, Calcium Green G 555/30 TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange Y 590/27 MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR R 631/33 Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N FR 735/40 Alexa Fluor 750, Alexa Fluor 790, Cy7, Cy7.5 X-Cite Xylis: The X-Cite Xylis XT720L is a broad spectrum illuminator with a range from 380-770nm.  It has peaks at 385, 430, 475, 545, 635, 735nm. Filters: Filter Cubes: In multi-band fluorescence imaging, the filter turret has to move between positions for each band.  This is usually done on a per-tile basis.  At about 400ms per move, this can add up, especially for a large scan at higher magnification.  The Axioscan 7 has 2 multi-band cubes.  If these can be used, it can reduce or eliminate these moves and reduce scan time.  These can be used on the Axioscan 7 because it has the Colibri 7 switching LED source. Filter No  Fluorochrome Excitation (nm) Beamsplitter (nm) Emission (nm) 1 Empty (Brightfield)       2 HE GFP 450-490 495 500-550 3 HE DsRed 538-562 570 570-640 4 Cy5 625-655 660 665-715 5 HE BFP 370-410 420 430-470 6 HE DAPI/GFP/Cy3.5/Cy7 375-395 455-483 583-600 720-750 405 493 611 762 410-440 501-547 618-650 770-800 7 HE DAPI/GFP/Cy3.5/Cy5/Cy7 370-400 450-488 540-570 614-647 720-750 405 493 575 654 761 412-438 501-527 582-601 662-700 770-800 Pol Circular polarizer and analyzer ND 6% Neutral Density Filter Fast Filter Wheel: The fast filter wheel moves between filter positions about 10 times faster than the filter cube turret. Need information here Filter Pass Peak and Width Typical fluorophores Empty (Brightfield) Single Channel 1 Single Channel 2 Single Channel 3 Dual Channel 4 Dual Channel 5 Objectives: Objective Magnification Immersion Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm) Fluar M27 5X Air 0.25 0.17 12.5 Pan Apo M27 10X Air 0.45 0.17 2.1 Pan Apo M27 20X Air 0.8 0.17 0.55 Pan Apo Korr M27 40X Air 0.95 0.13-0.21 0.25 EC Pan-Neofluar Pol M27 20X Air 0.5 0.17 2 Zeiss 880 Airy Scan Location:CSC Room 032 Main features of Zeiss 880 Airy Scan System: Five detectors, Three Photo Multipliers Tube (PMT) detectors and two GaSP detectors for enhanced sensitivity and dynamic range for 5 total fluorescent channels. Transmitted light detector available for simultaneous brightfield imaging of samples while scanning confocally. AIRY scan detector capable of super resolution imaging Fast AIRY scan system for high speed super resolution imaging timelapse Epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing. Filter wheel used for epifluorescence viewing of sample is currently outfitted with with DAPI (blue), FITC (green), and TRITC (red), filter cube sets. Laser autofocus system provides focal depth consistency across varying sample regions of interest. Piezo Z stage for fast z acquisition IBIDI stage top incubator with temperature, C02 and humidity. Plates and dishes with glass bottoms. Imaging lasers: 405nm diode laser 488nm Argon gas laser 561nm diode 638nm diode Suggested Applications: Multichannel fluorescence and transmitted fixed slide imaging Tiling mosaics of fixed or live samples Timelapse of dishes or glass bottom well plates Objectives: O bjective Magnification Immersion Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm) Plan Apo M27 5 Air 0.16 0.17 12.1 EC Plan-Neofluar M27 10 Air 0.3 0.17 5.2 Plan Apo M27 20 Air 0.8 0.17 0.55 Plan Apo M27 40 Oil 1.3 0.17 0.21 Plan Apo DIC M27 63 Oil 1.4 0.17 0.19 Calibration Apo DIC M27   StedyCon Location: CSC Room 30 Main features of StedyCon: STEDYCON from Abberior is a new class of nanoscope with multicolor confocal and STED (Stimulated emission depletion) capabilities. The lateral STED resolution could reach 30nm depending on dyes and objectives used. The acquired STEDYCON system is fitted on a Nikon Ti-S-E inverted microscope from a camera port and equipped with four excitation lasers: 405 nm CW and 488, 561, 640 nm pulse laser. The STED process is applied by a 775 nm pulse laser. Four highly sensitive single-photon-counting Avalanche photodiode detectors (APD) are mounted. Two STED and four confocal imaging channels can be detected.  A 100X/NA=1.45 oil immersion lens and other low magnification lens are provided to satisfy versatile measurements. An XY stage from Marzhauser and an ultra-fast and precise Piezo Z stage from PIFOC are mounted.  Browser-based acquisition software is installed on a powerful PC with a Windows 10 system. Other advanced functions including Z stack, multipositioning, time series, and tiling and stitching can be performed. Objectives: Objective Magnification Immersion  Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm) Plan Apo Lambda 4X Air 0.2 20 Okan-Neofluar 5X Air 0.15 0.17 13.6 Plan Apo 10X Air 0.45   0.17 4 PlanApo Lambda 20X Air 0.75 0.17 1 Plan Apo Lambda 60X Oil 1.4 0.17 0.13 Plan Apo Lambda 100x Oil 1.45 0.17 0.13   Olympus FV1000 Confocal Microscope Location: CSC Room 32  Main features of System: Three standard Photo Multipliers Tube (PMT) light detectors with two of the channels having tunable spectral bandwidths. Simultaneous transmitted light or DIC imaging while scanning confocally. Fully automated Olympus IX81 inverted microscope. Additional epifluorescence and DIC (Nomarski) illumination lamps for rapid sample identification and focusing. Motorized Prior II XY stage with a variety of stage inserts available (slides,dishes, multiwall plates, etc..). Olympus FV10-ASW 3.1 confocal imaging software Imaging lasers: 405nm diode laser 488nm Argon gas laser 543nm Helium-neon gas laser 633nm Helium-neon gas laser Objectives: Objective Magnification Immersion  Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm) UPlanSApo 10X Air 0.4 0.17 3.1 UPlanApo 20X Air 0.7 0.17 0.65 UApo 40X Oil 1.35 Corr 0.17 0.1 PlanApo 60X Water 1.2 Corr 0.13-0.21 0.28 PlanApo 60X Oil 1.4 0.17 0.12 Delta Vision Widefield Location: EEJMRB Room 5100 Hardware: Customized Olympus IX73 microscope base w/ TruLight Illuminator Olympus X-line PlanApo Objectives 4x, 10x, 20x Dry and 60x Oil Ultimate Focus laser-based autofocus system Custom XYZ Stage 106mm x 70mm x 7mm sCMOS Camera 2040 x 2040 pixels 7-channel solid state illuminator DIC Transmitted Light Imaging Environmental Chamber Primary applications: 2D and 3D Multi-Channel Fluorescence Imaging 3D Restorative Deconvolution Time-Lapse Imaging Multipoint Imaging Objectives: Objective Magnification Immersion Numerical Aperture Correction Ring Coverslip (mm) Working Distance (mm) UPlanXApo 4 Air 0.16 13 UPlanXApo 10 Air 0.4 0.17 3.1 UPlanXApo 20 Air 0.8 0.17 0.6 UPlanXApo 40 Oil 1.4 0.17 0.12 UPlanXApo 60 Oil 1.42 0.17 0.15 Leica Spinning Disk Confocal Location: EEJMRB Room 5122 Hardware: Leica DMi8 motorized microscope with adaptive focus tracking ASI motorized stage with Piezo Z W-1 large format spinning disk confocal with beam homogenizer Andor 1024 x 1024 EMCCD camera Visitron 2D FRAP unit 405,488,561,640nm laser lines for DAPI, GFP, RFP, Cy5, and similar fluorochromes W-View image splitter for dual channel simultaneous imaging LED based widefield fluorescence Stage top incubator system Visiview acquisition software Primary applications: 2D and 3D Multi-Channel Fluorescence Imaging 3D Restorative Deconvolution Time-Lapse Imaging Multipoint Imaging Sample images before (A) and after (B) deconvolution Objectives: Objective Magnification Immersion Numerical Aperture Correction Ring Coverglass (mm) Working Distance (mm) HC PL APO CS2 10 Air 0.4 2.74 HCX PL APO CS 20 Air 0.75 0.17 0.62 HC PL APO CS2 40 Water 1.1 Corr 0.14-0.18 0.65 HC PL APO CS2 63 Oil 1.4 0.17 0.14 Nikon Ring TIRF or Spinning Disk Confocal Location: EEJMRB Room 3532 Objective Magnification Immersion Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm) Plan Fluor 10 Air 0.3 0.17 16 Plan Fluor 10 Air 0.3 0.17 16 Plan Apo 20 Air 0.75 0.17 1 Apo TIRF 60 Oil 1.49 Corr 0.13-0.19 0.16-0.07 Plan Apo Lambda 60 Oil 1.49 0.17 0.13 Apo TIRF 100 Oil 1.49 Corr 0.13-0.19 0.16-0.09