Instrument Details

Details about each instrument

Zeiss 700 Confocal Microscope

Location: HSC (Building 586) Room 56

image-1700073367695.jpeg

The LSM 700 laser scanning confocal microscope is the seventh generation of confocal microscopes from Carl Zeiss. The LSM 700 system uses a Zeiss AXIO Observer Z1 inverted microscope with transmitted illumination (HAL 100) and a laser illumination source. The LSM 700 system features easy operation, excellent sensitivity, and a design that can handle complex tasks.

Main features of Zeiss 700 System:

Suggested Applications:

Imaging lasers:

Wavelength (nm) Type Power Manufacturer
405 diode 5 mW

Lasos Lasertechnik GmbH

488 diode 10 mW

Lasos Lasertechnik GmbH

555 diode 10 mW

Lasos Lasertechnik GmbH

639 diode 5 mW

Lasos Lasertechnik GmbH

Objectives:

Objective Magnification Immersion

Numerical

Apperature

Correction Ring Coverglass (mm)

Working

Distance (mm)

EC Plan Neofluar M27 10X Air 0.3
0.17

5.2

Pan Apo M27 20X Air 0.8
0.17 0.55
LD C-Apo Korr M27 40X Water 1.1 Corr 0.14-0.19 0.62
Pan Apo DIC M27 63X Oil 1.4
0.17 0.19

Prairie Ultima 2

Location: HSC Room 48B / 48D

Main features of Prairie Ultima 2/Multi-Photon Confocal System:

Imaging lasers:

Suggested Applications:

Objectives:

48B:
Objective Magnification Immersion Numerical Aperture Correction Collar Coverslip (mm) Working Distance (mm)
Apo 25 Water, Water Dipping 1.1 Corr 0-0.17 2
48D:
Objective Magnification Immersion Numerical Aperture Correction Collar Coverslip (mm) Working Distance (mm)
Plan Apo Lambda 4 Air 0.2

20

Plan Fluor 10 Air 0.3
0.17

16

UPlanFl 10 Air 0.3


LWD 16 Water Dipping 0.8
0

3

Apo 25 Water, Water Dipping 1.1 Corr 0-0.17

2

Fluor 40 Water Dipping 0.8
0

2

Nikon A1R Confocal

Location: HSC Room 56

Main features of System:

Imaging lasers:

Suggested Applications:

Objectives:

Objective Magnification Immersion Numerical Aperture Correction Ring Coverglass Working Distance
Plan Apo Lambda 4 Air 0.2

20
Plan Apo Lambda 10 Air 0.45
0.17 4
Plan Apo Lambda 20 Air 0.75
0.17 1
Plan Fluor 40 Oil 1.3
0.17 0.2
Plan Apo Lambda 60 Oil 1.4
0.17 0.13

Other objectives available. Please inquire with Core personnel

Evos Auto Color and Fluor Microscope

Location: HSC (Building 586) Room 54

CI-Evos.jpg

The EVOS FL Imaging system is an all-in-one fluorescence microscope designed for efficiency and ease of use. The on-board computer and integrated imaging software allows users to capture and save four channels fluorescence images/data directly from the microscope. The EVOS FL Imaging System is suitable for checking samples before using a high-end microscope system.

Main features of System:

Suggested Applications:

Light Sources:

LED light cubes which combine bright LED illumination with excitation and emission filters.  DAPI, CY5, GFP, RFP and Texas Red cubes are available.  Four can be installed at one time.  Contact Cell Imaging staff to change cubes if needed.  A selection guide for matching cubes to dyes is on the ThermoFisher site: Guide

Cube Excitation (nm) Emission (nm)
DAPI 357/44 447/60
GFP 485/25 524/24
RFP 531/40 593/40
Texas Red 585/29 628/32
Cy5 628/40 692/40

Objectives:

The objectives on the Evos are Long Working Distance, not Coverslip-Corrected.  They are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm.

Objective Magnification Immersion

Numerical

Apperature

Correction Ring Coverglass (mm)

Working

Distance (mm)

PlanApo 1.25X Air 0.04

5.10

Plan Fluor 4X Air 0.13

17.2

Plan Fluor 10X Air 0.3
1.2 8.31
Plan Fluor 20X Air 0.45
1.2 7.11
Plan Fluor 40X Air 0.65
1.2 2.81


Zeiss Axio Scan.Z1

Location: HSC (Building 586) Room 60

CI-ZeissAxioScanZ1.jpg

The Zeiss Axio-Scan.Z1 is a fast and flexible slide scanner that digitizes specimens to produce high-quality virtual slides. In addition to brightfield slide scanning, the Axio-Scan.Z1 uses LED lights to scan up to nine fluorescent channels. Up to 100 slides can be loaded onto the scanner, making it suitable for large projects, including digital archives. Scanned images are saved as CZI files and can be viewed using Zeiss freeware Zen-lite or third-party image analysis programs.

Main features of Axio Scan.Z1:

Light Sources:

In addition to an internal lamp for brightfield imaging, the Axio Scan.Z1 has an X-Cite 120LED source for fluorescent imaging.

Filter cubes for LEDs:

The scanner has a 10 position filter turret to fit most fluorophores.  The Zen profile wizard is used to pick filter to match your imaging needs.  Alternate filter cubes can be installed for specialty applications.  Please consult with Cell Imaging staff for consultation and assistance.

Filter Position Excitation (nm) Emission (nm) Typical Fluorophores Opal Dye
1 empty empty Brightfield
2 325-375 435-485 DAPI
3 426-446 465-495 Cyan, Protein 480
4 480-500 510-530 Alexa Fluor 488 520
5 510-530 540-560 Alexa Fluor 489 540
6 538-562 570-640 HE, DsRed, Alexa Fluor 555 570
7 568-592 610-640 Alexa Fluor 568 620
8 610-630 645-675 Alexa Fluor 660 650
9 625-655 666-715 Cy5
10 650-680 695-755 Alexa Fluor 680 690

Objectives:

Objective Magnification Immersion

Numerical

Apperature

Correction Ring Coverglass (mm)

Working

Distance (mm)

EC Plan-Neofluar M27 2.5X Air

0.085


0.17

8.8

Fluor M27 2.5X Air

0.12


0.17

8.7

Fluar M27 5X Air 0.25
0.17

12.5

Plan Apo M27 10X Air 0.45
0.17

2.1

Plan Apo M27 20X Air 0.8
0.17 0.55
Plan Apo Korr M27 40X Air 0.95
0.13-0.21 0.25

 

 

Nikon Automated Widefield Microscope

Location: HCI Room 1430

Main features of System:

Suggested Applications:

Objectives:

Objective Magnification Immersion Numerical Aperture Correction Ring Coverglass (mm) Working Distance (mm)
Plan Apo Lambda 4 Air 0.2

20
Plan Apo Lambda 10 Air 0.45

0.17

4
Plan Apo Lambda 20 Air 0.75
0.17 1
S PLAN Fluor ADM ELWD 20 Air 0.45 Corr 0-2.0 8.2-6.9
Plan Apo Lambda 60 Oil 1.4
0.17 0.13
Plan Apo Lambda 100 Oil 1.45
0.17 0.13

Other objectives available. Please inquire with Core personnel

Leica SP8 White Light System

Location: HCI Room 1480

Main features of Leica SP8 White Light System:

Imaging lasers:

Suggested Applications:

Objectives:

Objective Magnification Immersion Numerical Aperture Correction Ring Coverglass (mm) Working Distance (mm)
HC PL APO CS2 10 Air 0.4

2.74
HC PL APO CS2 20 Either water, glycerine, or oil 0.75 Corr
0.66
HC PL APO CS2 20 Air 0.75
0.17 0.62
HC PL APO CS2 40 Water 1.1 Corr 0.14-0.18 0.65
HC PL APO CS2 40 Oil 1.3
0.17 0.24
HC PL APO CS2 60 Oil 1.4
0.17 0.14

Other objectives available. Please inquire with Core personnel

Leica SP8 405-488-561-633 Laser Confocal

Location: HCI Room 1440

Main features of Leica SP8 White Light System:

Imaging lasers:

Suggested Applications:

Objectives:

Objective Magnification Immersion Numerical Aperture Correction Collar Coverslip (mm) Working Distance (mm)
HC PL APO CS2 10 Air 0.4

2.74
HC PL APO CS2 20 Either water, glycerine, or oil 0.75 Corr
0.66
HC PL APO CS2 20 Air 0.75
0.17 0.62
HC PL APO CS2 40 Water 1.1 Corr 0.14-0.18 0.65
HC PL APO CS2 40 Oil 1.3
0.17 0.24
HC PL APO CS2 63 Oil 1.4
0.17 0.14

Zeiss Axioscan 7

Location: HCI Room 1470

CI-Axio7.jpg

The Zeiss AxioScan 7 is a fast and flexible slide scanner that digitizes specimens to produce high-quality virtual slides. In addition to brightfield slide scanning, the Axioscan 7 can scan up to five fluorescent channels using fast switching LED lights. Up to 100 slides can be loaded onto the scanner, making it suitable for large projects, including digital archives. Scanned images are saved as CZI files and can be viewed using Zeiss freeware Zen-lite or third-party image analysis programs.

Main Features of the Axioscan 7:

Suggested Applications:

Configurations:

The Axioscan 7 has a replaceable filter turret and can be switched between two light sources.  The 10 position filter cube turret is typically used with the Colibri 7 switchable LED source.  The fast filter wheel is typically used with the X-Cite Xylis LED source.

Configuration Light Source Filter Set
1 Colibri 7 Filter Cubes
2 X-Cite Xylis Fast Filter Wheel

Only Cell Imaging staff are permitted to switch between configurations.   Please schedule our time and plan accordingly.

 Light Sources:

Colibri 7:

The Colibri 7 has 6 LEDs with excitation filters to provide 7 excitation bands.  (One LED has a motorized filter to split it into two bands.)

Excitation Bands (There is one too many here)

Band Wavelength / Bandwidth (nm) Some typical fluorophores
UV 385/30

API, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue

V 423/44

Pacific Blue, Lucifer Yellow, Alexa Fluor 433, eCFP, Cerulean

B 469/38

FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC / Fluorescein, Kaede (green / red), PerCP, YoYo-1,FuraRed

C 511/44

Rhodamine 123, Fluo-4, Oregon Green BAPTA, Sytox Green, eYFP, FM4-64, Eosin/HE, Acridine Orange, JC1, Bodipy FL, Propidium Iodide, Spectrum Green, Calcium Green

G 555/30

TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange

Y 590/27

MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR

R 631/33

Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N

FR 735/40

Alexa Fluor 750, Alexa Fluor 790, Cy7, Cy7.5

X-Cite Xylis:

The X-Cite Xylis XT720L is a broad spectrum illuminator with a range from 380-770nm.  It has peaks at 385, 430, 475, 545, 635, 735nm.

Filters:

Filter Cubes:

In multi-band fluorescence imaging, the filter turret has to move between positions for each band.  This is usually done on a per-tile basis.  At about 400ms per move, this can add up, especially for a large scan at higher magnification.  The Axioscan 7 has 2 multi-band cubes.  If these can be used, it can reduce or eliminate these moves and reduce scan time.  These can be used on the Axioscan 7 because it has the Colibri 7 switching LED source.

Filter No

 Fluorochrome

Excitation (nm)

Beamsplitter (nm)

Emission (nm)

1

Empty (Brightfield)

 

 

 

2

HE GFP

450-490

495

500-550

3

HE DsRed

538-562

570

570-640

4

Cy5

625-655

660

665-715

5

HE BFP

370-410

420

430-470

6

HE DAPI/GFP/Cy3.5/Cy7

375-395

455-483

583-600

720-750

405

493

611

762

410-440

501-547

618-650

770-800

7

HE DAPI/GFP/Cy3.5/Cy5/Cy7

370-400

450-488

540-570

614-647

720-750

405

493

575

654

761

412-438

501-527

582-601

662-700

770-800

Pol

Circular polarizer and analyzer




ND

6% Neutral Density Filter




Fast Filter Wheel:

The fast filter wheel moves between filter positions about 10 times faster than the filter cube turret.

Need information here

Filter Pass Peak and Width Typical fluorophores
Empty (Brightfield)

Single Channel 1

Single Channel 2

Single Channel 3

Dual Channel 4

Dual Channel 5

Objectives:

Objective Magnification Immersion

Numerical

Apperature

Correction Ring Coverglass (mm)

Working

Distance (mm)

Fluar M27 5X Air 0.25
0.17

12.5

Pan Apo M27 10X Air 0.45
0.17 2.1
Pan Apo M27 20X Air 0.8
0.17 0.55
Pan Apo Korr M27 40X Air 0.95
0.13-0.21 0.25
EC Pan-Neofluar Pol M27 20X Air 0.5
0.17 2


Zeiss 880 Airy Scan

Location:CSC Room 032

CI-Zeiss880.jpeg

Main features of Zeiss 880 Airy Scan System:

Imaging lasers:

Suggested Applications:

Objectives:

Objective

Magnification Immersion Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm)
Plan Apo M27 5 Air 0.16
0.17 12.1
EC Plan-Neofluar M27 10 Air 0.3
0.17 5.2
Plan Apo M27 20 Air 0.8
0.17 0.55
Plan Apo M27 40 Oil 1.3
0.17 0.21
Plan Apo DIC M27 63 Oil 1.4
0.17 0.19
Calibration Apo DIC M27
 




StedyCon

Location: CSC Room 30

Main features of StedyCon:

Objectives:

Objective Magnification Immersion  Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm)
Plan Apo Lambda 4X Air 0.2

20
Okan-Neofluar 5X Air 0.15
0.17 13.6
Plan Apo 10X Air 0.45   0.17 4
PlanApo Lambda 20X Air 0.75
0.17 1
Plan Apo Lambda 60X Oil 1.4
0.17 0.13
Plan Apo Lambda 100x Oil 1.45
0.17 0.13

 

Olympus FV1000 Confocal Microscope

Location: CSC Room 32 

Main features of System:

Imaging lasers:

Objectives:

Objective Magnification Immersion  Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm)
UPlanSApo 10X Air 0.4
0.17 3.1
UPlanApo 20X Air 0.7
0.17 0.65
UApo 40X Oil 1.35 Corr 0.17 0.1
PlanApo 60X Water 1.2 Corr 0.13-0.21 0.28
PlanApo 60X Oil 1.4
0.17 0.12


Delta Vision Widefield

Location: EEJMRB Room 5100

CI-Deltavision.png

Hardware:

Primary applications:

Objectives:

Objective Magnification Immersion Numerical Aperture Correction Ring Coverslip (mm) Working Distance (mm)
UPlanXApo 4 Air 0.16

13
UPlanXApo 10 Air 0.4
0.17

3.1

UPlanXApo 20 Air 0.8
0.17

0.6

UPlanXApo 40 Oil 1.4
0.17

0.12

UPlanXApo 60 Oil 1.42
0.17

0.15


Leica Spinning Disk Confocal

Location: EEJMRB Room 5122

Hardware:

Primary applications:

Objectives:

Objective Magnification Immersion Numerical Aperture Correction Ring Coverglass (mm) Working Distance (mm)
HC PL APO CS2 10 Air 0.4

2.74
HCX PL APO CS 20 Air 0.75
0.17 0.62
HC PL APO CS2 40 Water 1.1 Corr 0.14-0.18 0.65
HC PL APO CS2 63 Oil 1.4
0.17 0.14


Nikon Ring TIRF or Spinning Disk Confocal

Location: EEJMRB Room 3532


Objective Magnification Immersion Numerical Apperature Correction Ring Coverglass (mm) Working Distance (mm)
Plan Fluor 10 Air 0.3
0.17 16
Plan Fluor 10 Air 0.3
0.17 16
Plan Apo 20 Air 0.75
0.17 1
Apo TIRF 60 Oil 1.49 Corr 0.13-0.19 0.16-0.07
Plan Apo Lambda 60 Oil 1.49
0.17 0.13
Apo TIRF 100 Oil 1.49 Corr 0.13-0.19 0.16-0.09